Oligonucleotide sequences of RT-PCR primers
Gene . | Forward primer . | Reverse primer . |
|---|---|---|
| β-actin | CTTTGCAGCTCCTTCGTTGC | TCTGACCCATTCCCACCATCAC |
| HPRT | CCAGCGTCGTGATTAGCGATG | ATAGCCCCCCTTGAGCACACAGAG |
| βmajor-globin | GCACCTGACTGATGCTGAGAAGG | TGGGTCCAAGGGTAGACAACC |
| α-globin | TGCTCTCTGGGGAAGACAAAAG | CGTGGCTTACATCAAAGTGAGGG |
| Fli1 | AACGAACGGAGAGTCATTGTGC | TTCTGTGTTGTAGGCGGAGGTG |
| EpoR | TGACGCTGTCTCTCATTCTGGTCC | TCAAACTCGCTCTCTGGGCTTG |
| Runx1 | CAGGCAACGATGAAAACTAC | GCTCGGAAAAGGACAAAC |
| GATA1 | CCCTTGTGAGGCCAGAGA | ACCTGATGGAGCTTGAAATAG |
| GATA2 | GTCAGACGACAACCACCACCTTA | GGATTTGCTGGACATCTTCCGA |
| PU.1 | TGGAAGGGTTTTCCCTCACC | TGCTGTCCTTCATGTCGCCG |
| SCL | CTCCTATCACATTCTGCTATCAC | AATCTCTCACCCCTTCTCAC |
| c-fms | TAGGTGTGATGAAGCCCAG | TCAGTGATGACCAGGACAG |
| c-Kit | AAGGGGACACATTTACGGTGG | TCCAGAATCGTCAACTCTTGCC |
Gene . | Forward primer . | Reverse primer . |
|---|---|---|
| β-actin | CTTTGCAGCTCCTTCGTTGC | TCTGACCCATTCCCACCATCAC |
| HPRT | CCAGCGTCGTGATTAGCGATG | ATAGCCCCCCTTGAGCACACAGAG |
| βmajor-globin | GCACCTGACTGATGCTGAGAAGG | TGGGTCCAAGGGTAGACAACC |
| α-globin | TGCTCTCTGGGGAAGACAAAAG | CGTGGCTTACATCAAAGTGAGGG |
| Fli1 | AACGAACGGAGAGTCATTGTGC | TTCTGTGTTGTAGGCGGAGGTG |
| EpoR | TGACGCTGTCTCTCATTCTGGTCC | TCAAACTCGCTCTCTGGGCTTG |
| Runx1 | CAGGCAACGATGAAAACTAC | GCTCGGAAAAGGACAAAC |
| GATA1 | CCCTTGTGAGGCCAGAGA | ACCTGATGGAGCTTGAAATAG |
| GATA2 | GTCAGACGACAACCACCACCTTA | GGATTTGCTGGACATCTTCCGA |
| PU.1 | TGGAAGGGTTTTCCCTCACC | TGCTGTCCTTCATGTCGCCG |
| SCL | CTCCTATCACATTCTGCTATCAC | AATCTCTCACCCCTTCTCAC |
| c-fms | TAGGTGTGATGAAGCCCAG | TCAGTGATGACCAGGACAG |
| c-Kit | AAGGGGACACATTTACGGTGG | TCCAGAATCGTCAACTCTTGCC |