ATP-induced MMP-9 release in monocytes and T lymphocytes
. | Unpurified PBMCs . | . | Positively selected monocytes . | . | Positively selected T lymphocytes . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Subject . | Basal . | ATP . | Basal . | ATP . | Basal . | ATP . | |||
| 1 | 1.1 | 6.7 | 8.9 | 12.5 | 0.7 | 0.6 | |||
| 2 | 1.8 | 14.5 | 2.9 | 3.5 | 0.5 | 0.4 | |||
| 3 | 4.3 | 12.0 | 24.8 | 30.1 | 0.1 | 0.1 | |||
| 4 | 11.2 | 34.5 | 12.3 | 12.2 | 0.8 | 0.6 | |||
. | Unpurified PBMCs . | . | Positively selected monocytes . | . | Positively selected T lymphocytes . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Subject . | Basal . | ATP . | Basal . | ATP . | Basal . | ATP . | |||
| 1 | 1.1 | 6.7 | 8.9 | 12.5 | 0.7 | 0.6 | |||
| 2 | 1.8 | 14.5 | 2.9 | 3.5 | 0.5 | 0.4 | |||
| 3 | 4.3 | 12.0 | 24.8 | 30.1 | 0.1 | 0.1 | |||
| 4 | 11.2 | 34.5 | 12.3 | 12.2 | 0.8 | 0.6 | |||
Monocytes and T cells from healthy subjects with wild-type P2X7 were purified by positive selection using MACS beads. Purified monocytes (2 × 107/mL), purified T cells (2 × 107/mL), and PBMCs (2 × 107/mL) were incubated with or without 1 mM ATP for 60 minutes. Supernatants were collected, and MMP-9 concentrations (ng/mL) were measured by ELISA (as shown in the table).