ATP-induced MMP-9 release in monocytes and T lymphocytes
. | Unpurified PBMCs . | . | Negatively selected monocytes . | . | Negatively selected T lymphocytes . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Subject . | Basal . | ATP . | Basal . | ATP . | Basal . | ATP . | |||
| 5 | 1.1 | 5.1 | 23.1 | 39.8 | 0 | 0.2 | |||
| 6 | 6.5 | 11.2 | 41.1 | 73.4 | 0.6 | 1.5 | |||
| 7 | 30.8 | 46.7 | 126.7 | 183.1 | 4.0 | 6.2 | |||
. | Unpurified PBMCs . | . | Negatively selected monocytes . | . | Negatively selected T lymphocytes . | . | |||
|---|---|---|---|---|---|---|---|---|---|
| Subject . | Basal . | ATP . | Basal . | ATP . | Basal . | ATP . | |||
| 5 | 1.1 | 5.1 | 23.1 | 39.8 | 0 | 0.2 | |||
| 6 | 6.5 | 11.2 | 41.1 | 73.4 | 0.6 | 1.5 | |||
| 7 | 30.8 | 46.7 | 126.7 | 183.1 | 4.0 | 6.2 | |||
Monocytes and T cells from healthy subjects with wild-type P2X7 were purified by negative selection using MACS beads. Purified monocytes (2 × 107/mL), purified T cells (2 × 107/mL), as well as PBMCs (2 × 107/mL) were incubated with or without 1 mM ATP for 60 minutes. Supernatants were collected, and MMP-9 concentrations (ng/mL) were measured by ELISA (as shown in the table).