Kinetics of recovery of various CFUs in BM-MNCs after TBI
Day after TBI . | CFU-GM . | BFU-E . | CFU-GEMM . |
|---|---|---|---|
| CTL | 66.8 ± 8.8 | 12.7 ± 2.3 | 3.3 ± 1.6 |
| 1 | 5.7 ± 1.0 | 0.2 ± 0.4 | nd |
| 3 | 8.0 ± 2.4 | 1.2 ± 1.2 | 0.3 ± 0.4 |
| 7 | 14.8 ± 8.5 | 1.5 ± 0.5 | 0.8 ± 0.9 |
| 14 | 27.0 ± 13.4 | 4.0 ± 3.2 | 1.5 ± 0.5 |
| 28 | 33.3 ± 8.7 | 8.7 ± 4.5 | 1.0 ± 1.0 |
| 56 | 40.4 ± 8.2 | 8.6 ± 3.6 | 1.7 ± 0.8 |
Day after TBI . | CFU-GM . | BFU-E . | CFU-GEMM . |
|---|---|---|---|
| CTL | 66.8 ± 8.8 | 12.7 ± 2.3 | 3.3 ± 1.6 |
| 1 | 5.7 ± 1.0 | 0.2 ± 0.4 | nd |
| 3 | 8.0 ± 2.4 | 1.2 ± 1.2 | 0.3 ± 0.4 |
| 7 | 14.8 ± 8.5 | 1.5 ± 0.5 | 0.8 ± 0.9 |
| 14 | 27.0 ± 13.4 | 4.0 ± 3.2 | 1.5 ± 0.5 |
| 28 | 33.3 ± 8.7 | 8.7 ± 4.5 | 1.0 ± 1.0 |
| 56 | 40.4 ± 8.2 | 8.6 ± 3.6 | 1.7 ± 0.8 |
Mice were exposed to 6.5 Gy TBI or unirradiated as control (CTL). At 1, 3, 7, 14, 28, and 56 days after TBI, BM-MNCs were isolated from irradiated and unirradiated mice. A CFC assay was performed. The results are presented as mean CFUs per 5 × 104 BM-MNCs ± SE (n = 3). ANOVA analysis revealed that TBI caused a significant reduction in the frequencies of CFU-GM, BFU-E, and CFU-GEMM in BM-MNCs in a time-dependent manner compared with the control values (P < .001). nd indicates not detectable.