Quantification of λ light chain secretion by multiple myeloma cell lines
Cell line
.
Secreted λ, μg/1.0 × 106 cells
.
8226/S
29.96 ± 7.39
MM.1S
15.05 ± 4.84
Cell line
.
Secreted λ, μg/1.0 × 106 cells
.
8226/S
29.96 ± 7.39
MM.1S
15.05 ± 4.84
MM cells were cultured at 5.0 × 105 cells/mL for 24 hours, after which the volume of media containing 1.0 × 106 cells was removed from the culture. Cells were pelleted, and the supernatant was removed for ELISA analysis. The data represent the mean ± the SD from 3 independent experiments.