Quantitative RT-PCR results for ORF 50 and ORF 26 in BC3 cells
. | Fold increase in mRNA . | . | |
|---|---|---|---|
. | ORF 50 . | ORF 26 . | |
| Butyrate | 42.8 | 400.2 | |
| TPA | 12.6 | 100.1 | |
| Doxycycline | 1.1 | 0.8 | |
| Butyrate + doxycycline | 85.4 | 812.3 | |
| TPA + doxycycline | 17.4 | 125.1 | |
. | Fold increase in mRNA . | . | |
|---|---|---|---|
. | ORF 50 . | ORF 26 . | |
| Butyrate | 42.8 | 400.2 | |
| TPA | 12.6 | 100.1 | |
| Doxycycline | 1.1 | 0.8 | |
| Butyrate + doxycycline | 85.4 | 812.3 | |
| TPA + doxycycline | 17.4 | 125.1 | |
BC3 cells were treated for 48 hours with butyrate (2 mM), TPA (20 ng/mL), doxycycline (2 μg/mL), or combinations as shown. Results are an average of 2 independent experiments and indicate that doxycycline has no independent negative effect on induction of ORF 50 or ORF 26.