IFNγ levels in APC cultures with MSCs pretreated with anti-IFNγRI
Cells/pretreated MSCs . | IFNγ, pg/mL . |
|---|---|
| Unpulsed MSCs | |
| Isotype | 20 ± 4 |
| Anti-IFNγRI | 22 ± 2 |
| Pulsed MSCs | |
| Isotype | 120 ± 8 |
| Anti-IFNγRI | 30 ± 3 |
| Activated CD4+ | |
| None | 88 ± 7 |
| Unactivated CD4+ | |
| None | 5 ± 0.5 |
| Unpulsed MSCs + unactivated CD4+ | |
| Isotype | 18 ± 3 |
| Anti-IFNγRI | 15 ± 2 |
| Unpulsed MSCs + activated CD4+ | |
| Isotype | 92 ± 5 |
| Anti-IFNγRI | 78 ± 5 |
| Pulsed MSCs + unactivated CD4+ | |
| Isotype | 160 ± 5 |
| Anti-IFNγRI | 28 ± 3 |
| Pulsed MSCs + activated CD4+ | |
| Isotype | 385 ± 12* |
| Anti-IFNγRI | 60 ± 6* |
Cells/pretreated MSCs . | IFNγ, pg/mL . |
|---|---|
| Unpulsed MSCs | |
| Isotype | 20 ± 4 |
| Anti-IFNγRI | 22 ± 2 |
| Pulsed MSCs | |
| Isotype | 120 ± 8 |
| Anti-IFNγRI | 30 ± 3 |
| Activated CD4+ | |
| None | 88 ± 7 |
| Unactivated CD4+ | |
| None | 5 ± 0.5 |
| Unpulsed MSCs + unactivated CD4+ | |
| Isotype | 18 ± 3 |
| Anti-IFNγRI | 15 ± 2 |
| Unpulsed MSCs + activated CD4+ | |
| Isotype | 92 ± 5 |
| Anti-IFNγRI | 78 ± 5 |
| Pulsed MSCs + unactivated CD4+ | |
| Isotype | 160 ± 5 |
| Anti-IFNγRI | 28 ± 3 |
| Pulsed MSCs + activated CD4+ | |
| Isotype | 385 ± 12* |
| Anti-IFNγRI | 60 ± 6* |
MSCs from 4 different donors were subjected to overnight incubation with 0.1 to 1 μg/mL IFNγRI. Cells were washed and then pulsed with C albicans or T toxoid. After this, the MSCs were washed and then placed in APC cultures in the presence of anti-IFNγRI (0.1-1 μg/mL) or isotype control. After 24 hours, culture supernatants were quantitated for IFNγ by ELISA. The data are shown for values at 1 μg/mL anti-IFNγRI.
These values showed significant (P < .05) differences between anti-IFNγRI and isotype control.