Reactivity and deduced amino acid sequences of the insert from positive phage particles
. | Phage particle clones* . | . | . | ||
|---|---|---|---|---|---|
| . | Reactivity with mAb, A492nm‡ . | . | . | ||
| PDPL and denomination† . | Rituximab . | Infliximab . | Deduced amino acid sequences§ . | ||
| c7c | |||||
| R2-C | 2.07 | 0.164 | acWASNPSLc | ||
| R3-C | 2.97 | 0.125 | acPYSNPSLc | ||
| R7-C | 2.25 | 0.155 | acPFANPSTc | ||
| R8-, R12-, and R18-C | 2.24 ± 0.24∥ | 0.2 ± 0.05∥ | acNFSNPSLc | ||
| R10-C | 1.572 | 0.154 | acEHSNPSLc | ||
| R13-C | 1.542 | 0.176 | acWAANPSMc | ||
| R14-C | 1.93 | 0.135 | acPFSNPSMc | ||
| R15-C | 2.82 | 0.206 | acPYANPSLc | ||
| R16-C | 1.102 | 0.144 | acSWANPSQc | ||
| R17-C | 2.65 | 0.26 | acMFSNPSLc | ||
| R19-C | 2.74 | 0.177 | acPFANPSMc | ||
| ---aNPS | |||||
| Consensus | – | – | ---s | ||
| CD20 sequence | 166NCEPANPSEKNSP177 | ||||
| 7-mer | |||||
| R1-, R2-, R4-, R7-, R8-, R11-, R14-, R19-, and R20-L | 2.96 ± 0.36∥ | 0.012 ± 0.004∥ | ------WPRWLEN-- | ||
| 12-mer | |||||
| R3-L | 2.85 | 0.04 | -EGHEHQTSWWEL | ||
| R5-, R9-, R12-, R13-, and R15-L | 2.63 ± 0.24∥ | 0.011 ± 0.005∥ | QDKLTQWPKWLE | ||
| R6-L | 3 | 0.08 | HTSVERWPLWLE | ||
| R10-L | 2.61 | 0.03 | ---ITPWPHWLERSS | ||
| R18- and R20-L | 2.98 ± 0.19∥ | 0.059 ± 0.002∥ | TTTTSVWPAWLE | ||
| Consensus | – | – | ------wp-WlE | ||
. | Phage particle clones* . | . | . | ||
|---|---|---|---|---|---|
| . | Reactivity with mAb, A492nm‡ . | . | . | ||
| PDPL and denomination† . | Rituximab . | Infliximab . | Deduced amino acid sequences§ . | ||
| c7c | |||||
| R2-C | 2.07 | 0.164 | acWASNPSLc | ||
| R3-C | 2.97 | 0.125 | acPYSNPSLc | ||
| R7-C | 2.25 | 0.155 | acPFANPSTc | ||
| R8-, R12-, and R18-C | 2.24 ± 0.24∥ | 0.2 ± 0.05∥ | acNFSNPSLc | ||
| R10-C | 1.572 | 0.154 | acEHSNPSLc | ||
| R13-C | 1.542 | 0.176 | acWAANPSMc | ||
| R14-C | 1.93 | 0.135 | acPFSNPSMc | ||
| R15-C | 2.82 | 0.206 | acPYANPSLc | ||
| R16-C | 1.102 | 0.144 | acSWANPSQc | ||
| R17-C | 2.65 | 0.26 | acMFSNPSLc | ||
| R19-C | 2.74 | 0.177 | acPFANPSMc | ||
| ---aNPS | |||||
| Consensus | – | – | ---s | ||
| CD20 sequence | 166NCEPANPSEKNSP177 | ||||
| 7-mer | |||||
| R1-, R2-, R4-, R7-, R8-, R11-, R14-, R19-, and R20-L | 2.96 ± 0.36∥ | 0.012 ± 0.004∥ | ------WPRWLEN-- | ||
| 12-mer | |||||
| R3-L | 2.85 | 0.04 | -EGHEHQTSWWEL | ||
| R5-, R9-, R12-, R13-, and R15-L | 2.63 ± 0.24∥ | 0.011 ± 0.005∥ | QDKLTQWPKWLE | ||
| R6-L | 3 | 0.08 | HTSVERWPLWLE | ||
| R10-L | 2.61 | 0.03 | ---ITPWPHWLERSS | ||
| R18- and R20-L | 2.98 ± 0.19∥ | 0.059 ± 0.002∥ | TTTTSVWPAWLE | ||
| Consensus | – | – | ------wp-WlE | ||
–indicates not applicable.
Positive phage particles were isolated by panning with rituximab the disulfide constrained 7-mer (c7c) and linear 7- and 12-mer PDPLs
Rituximab (R)-specific phage clones expressing cyclic (C) or linear (L) peptides
Reactivity of phage particles supernatant with mAb as measured by ELISA. Supernatants from phage particles expressing cyclic and linear peptides were diluted 8 and 32 times, respectively
Multiple alignment was performed with MULTALIN at Pole Bio-Informatique Lyonnais.25 Flanking and motif amino acids are shown in small and bold letters respectively
Mean ± SD