Table 1. Primer sequences for... | American Society of Hematology

Table 1.

Primer sequences for amplification of the complete tumor immunoglobulin genes

Primer	Sequence 5′-3′	Application
DFDP47	GAGTCGACCATGGGAGGTGCAGCTGTTGGAGTCTGG	Rearrangement-specific VH primer for patients 1, 4
DFVH3	GAGTCGACCATGGGAGGTGCAGCTGGTGGAGTCTGG	Rearrangement-specific VH primer for patients 2, 3
DFDP71	TAGAGACCCATGGCAGGTGCAGCTGCAGTCG	Rearrangement-specific VH primer for patient 5
DFDP75/10	CAGTAGTCCATGGCAGGTGCAGCTGGTGCAGTC	Rearrangement-specific VH primer for patients 6, 7
DFJH3	CAGTAGACTCGAGGAAGAGACGGTGACCATTGTCCCTTGG	Rearrangement-specific JH primer for patient 3
DFX2JH4b	GAGTCGACTCGAGGAGGAGACGGTGACCAGGGTTCC	Rearrangement-specific JH primer for patients 1, 2, 4
DFJH4d	GACAGCACTCGAGGAGGAGACGGTGACCAGGG	Rearrangement-specific JH primer for patient 6
DFJH6b	CAGTAGACTCGAGGAGACGGTGACCGTGGTC	Rearrangement-specific JH primer for patient 5
DFDPK20/22	AGCTGAGGTCGACGGAAATTGTGTTGACGCAGTCTCCAG	Rearrangement-specific VK primer for patients 1, 7
DFDPK21	AGCTGAGGTCGACGGAAATAGTGATGACGCAGTCTCC	Rearrangement-specific VK primer for patient 3
DFSVK4	CTCAGCTGTCGACGGACATCGTGATGACCCAGTCTC	Rearrangement-specific VK primer for patient 2
DFS2DPK8	AGCTGAGGTCGACGGACATCCAGTTGACCCAGTCTCC	Rearrangement-specific VK primer for patient 4
DFJK1.2	GAGTCGAGCGGCCGCTTTGATTTCCACCTTGGTCCCTTG	Rearrangement-specific JK primer for patients 1-3, 7
DFJK4.2	GAGTCGAGCGGCCGCTTTGATCTCCACCTTGGTCCC	Rearrangement-specific JK primer for patient 4
DF2b2	CTCTACAGTCGACGCAGTCTGCCCTGACTCAGCCTGC	Rearrangement-specific VL primer for patient 5
DF31	GTCTACTGTCGACGTCTTCTGAGCTGACTCAGGACC	Rearrangement-specific VL primer for patient 6
DFJL3	TTGTATTGCGGCCGCTAGGACGGTCAGCTTGGTC	Rearrangement-specific JL primer for patient 5
DFJL1	ATTGACTGCGGCCGCTAGGACGGTGACCTTGGTC	Rearrangement-specific JL primer for patient 6

Primer	Sequence 5′-3′	Application
DFDP47	GAGTCGACCATGGGAGGTGCAGCTGTTGGAGTCTGG	Rearrangement-specific VH primer for patients 1, 4
DFVH3	GAGTCGACCATGGGAGGTGCAGCTGGTGGAGTCTGG	Rearrangement-specific VH primer for patients 2, 3
DFDP71	TAGAGACCCATGGCAGGTGCAGCTGCAGTCG	Rearrangement-specific VH primer for patient 5
DFDP75/10	CAGTAGTCCATGGCAGGTGCAGCTGGTGCAGTC	Rearrangement-specific VH primer for patients 6, 7
DFJH3	CAGTAGACTCGAGGAAGAGACGGTGACCATTGTCCCTTGG	Rearrangement-specific JH primer for patient 3
DFX2JH4b	GAGTCGACTCGAGGAGGAGACGGTGACCAGGGTTCC	Rearrangement-specific JH primer for patients 1, 2, 4
DFJH4d	GACAGCACTCGAGGAGGAGACGGTGACCAGGG	Rearrangement-specific JH primer for patient 6
DFJH6b	CAGTAGACTCGAGGAGACGGTGACCGTGGTC	Rearrangement-specific JH primer for patient 5
DFDPK20/22	AGCTGAGGTCGACGGAAATTGTGTTGACGCAGTCTCCAG	Rearrangement-specific VK primer for patients 1, 7
DFDPK21	AGCTGAGGTCGACGGAAATAGTGATGACGCAGTCTCC	Rearrangement-specific VK primer for patient 3
DFSVK4	CTCAGCTGTCGACGGACATCGTGATGACCCAGTCTC	Rearrangement-specific VK primer for patient 2
DFS2DPK8	AGCTGAGGTCGACGGACATCCAGTTGACCCAGTCTCC	Rearrangement-specific VK primer for patient 4
DFJK1.2	GAGTCGAGCGGCCGCTTTGATTTCCACCTTGGTCCCTTG	Rearrangement-specific JK primer for patients 1-3, 7
DFJK4.2	GAGTCGAGCGGCCGCTTTGATCTCCACCTTGGTCCC	Rearrangement-specific JK primer for patient 4
DF2b2	CTCTACAGTCGACGCAGTCTGCCCTGACTCAGCCTGC	Rearrangement-specific VL primer for patient 5
DF31	GTCTACTGTCGACGTCTTCTGAGCTGACTCAGGACC	Rearrangement-specific VL primer for patient 6
DFJL3	TTGTATTGCGGCCGCTAGGACGGTCAGCTTGGTC	Rearrangement-specific JL primer for patient 5
DFJL1	ATTGACTGCGGCCGCTAGGACGGTGACCTTGGTC	Rearrangement-specific JL primer for patient 6

VH indicates variable segments of the heavy-chain gene; VL, variable segment of the lambda light chain gene; VK, variable segment of the kappa light chain gene; JH, joining segment of the kappa light chain gene; JK, joining segment of the kappa light chain gene; and JL, joining segment of the lambda light chain gene.

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