Overview of the 23 normal human tissues (paraffin-embedded and/or frozen sections) stained with the tumor scFv's and controls, and the resulting staining patterns
. | Staining pattern . | . | |
|---|---|---|---|
| Tissue . | Ber-H2 scFv . | Patient 1 scFv . | |
| Tonsil* | Lymphoid blasts | Plasma cell subpopulation | |
| Spleen† | – | – | |
| Salivary gland‡ | – | – | |
| Esophagus‡ | – | – | |
| Stomach§ | – | – | |
| Small intestine∥ | Lymphoid blasts | – | |
| Duodenum‡ | – | – | |
| Colon¶ | – | – | |
| Appendix‡ | – | – | |
| Kidney | – | – | |
| Adrenal gland‡ | – | – | |
| Testis | – | – | |
| Prostate‡ | – | – | |
| Uterus‡ | – | – | |
| Ovary‡ | – | – | |
| Mammary# | – | – | |
| Placenta# | – | – | |
| Thyroid** | – | – | |
| Nerve‡ | – | – | |
| Liver | – | – | |
| Lung | – | – | |
| Skeletal muscle‡ | – | – | |
| Skin | – | – | |
. | Staining pattern . | . | |
|---|---|---|---|
| Tissue . | Ber-H2 scFv . | Patient 1 scFv . | |
| Tonsil* | Lymphoid blasts | Plasma cell subpopulation | |
| Spleen† | – | – | |
| Salivary gland‡ | – | – | |
| Esophagus‡ | – | – | |
| Stomach§ | – | – | |
| Small intestine∥ | Lymphoid blasts | – | |
| Duodenum‡ | – | – | |
| Colon¶ | – | – | |
| Appendix‡ | – | – | |
| Kidney | – | – | |
| Adrenal gland‡ | – | – | |
| Testis | – | – | |
| Prostate‡ | – | – | |
| Uterus‡ | – | – | |
| Ovary‡ | – | – | |
| Mammary# | – | – | |
| Placenta# | – | – | |
| Thyroid** | – | – | |
| Nerve‡ | – | – | |
| Liver | – | – | |
| Lung | – | – | |
| Skeletal muscle‡ | – | – | |
| Skin | – | – | |
The paraffin-embedded tissues were assembled as a tissue array (Biochip; Prof A. C. Feller, Institute of Pathology, Campus Lübeck, Universitatsklinikum Schleswig Holstein, Germany); in addition, single sections of tonsil, spleen, small intestine, and thyroid were stained. “Nonspecific staining” refers to signals detectable in the negative control (no antibody) and with the Ber-H2 control scFv, respectively.
No specific signals were found for patients 2 through 7 or for the negative control.
– Indicates no specific signals.
Nonspecific staining of single plasma cells or granulocytes in surrounding muscle
Nonspecific staining of single lymphatic cells
Stained as paraffin-embedded tissue only
Nonspecific staining of single glandular cells and of single plasma cells and granulocytes in the surrounding muscle
Nonspecific staining of single plasma cells in the glandular tissue and of single plasma cells and granulocytes in the surrounding muscle
Nonspecific staining of single glandular cells and of single plasma cells in the glandular tissue
Stained as frozen tissue only
Nonspecific staining of thyroglobulin