Rescue of MTAP+ primary T-ALL from L-alanosine toxicity
. | MTAP exon 8* . | MTAP RNA* . | MTAP protein . | 3H-thymidine incorporation, % of control . | . | |
|---|---|---|---|---|---|---|
| Patient . | . | . | . | Without EFA . | With EFA . | |
| Normal PBMCs | ND | + | + | 37.8 ± 2.3 | 101 ± 4.0 | |
| 061219 | + | + | + | 51.7 ± 1.7 | 82.2 ± 2.2 | |
| 220112 | + | + | + | 58.1 ± 4.0 | 99.9 ± 10.4 | |
| 130312 | + | + | + | 57.1 ± 7.1 | 89.4 ± 4.6 | |
| 131518-R | + | ND | ND | 72.6 ± 2.6 | 92.5 ± 8.1 | |
| 190114 | – | – | – | 3.4 ± 0.8 | 5.1 ± 1.0 | |
| 122614-R | + | + | – | 12.9 ± 1.3 | 37.6 ± 2.6 | |
| 260512 | + | + | ND | 14.4 ± 0.9 | 24.1 ± 0.8 | |
. | MTAP exon 8* . | MTAP RNA* . | MTAP protein . | 3H-thymidine incorporation, % of control . | . | |
|---|---|---|---|---|---|---|
| Patient . | . | . | . | Without EFA . | With EFA . | |
| Normal PBMCs | ND | + | + | 37.8 ± 2.3 | 101 ± 4.0 | |
| 061219 | + | + | + | 51.7 ± 1.7 | 82.2 ± 2.2 | |
| 220112 | + | + | + | 58.1 ± 4.0 | 99.9 ± 10.4 | |
| 130312 | + | + | + | 57.1 ± 7.1 | 89.4 ± 4.6 | |
| 131518-R | + | ND | ND | 72.6 ± 2.6 | 92.5 ± 8.1 | |
| 190114 | – | – | – | 3.4 ± 0.8 | 5.1 ± 1.0 | |
| 122614-R | + | + | – | 12.9 ± 1.3 | 37.6 ± 2.6 | |
| 260512 | + | + | ND | 14.4 ± 0.9 | 24.1 ± 0.8 | |
Primary T-All cells were plated at 0.5 × 106 cells/mL and cultured for 3 days in complete RPMI alone (control) or in complete RPMI containing 40 μM L-alanosine and increasing concentrations of EFA up to 40 μM. Cells were then pulsed with 3H-thymidine for 6 hours and incorporation of 3H-thymidine was determined in a scintillation counter. All culture conditions were performed in triplicate and error bars represent standard deviation. Patients 190114, 122614-R, and 260512 had MTAP-deficient and the other patients had MTAP-abundant cells. MTAP status is based on MTAP protein expression and activity as demonstrated by rescue from L-alanosine toxicity by EFA.
ND indicates not determined
Determined by PCR