Primers for real-time PCR analysis of ChIP material
Target region . | Central CACC site sequence . | Product size, bp, or reference . | Primer sequences . |
|---|---|---|---|
| β-Major globin | CCACACCCT | Letting et al37 | Forward: 5′-GAC AAA CAT TAT TCA GAG GGA GTA CCC |
| Reverse: 5′-AGG TGC ACC ATG ATG TAT GTT TAT GG | |||
| Nectin | N/A | Letting et al37 | Forward: 5′-TTT ACA TAA GCC TAG TGG TAC CCT |
| Reverse: 5′-ATC GCT GTC CTG CAT CTC ACA GTC G | |||
| ECR a | TGGGTGCGGC | 139 | Forward: 5′-AAT GAG AGG GTG GGT GGT TT |
| Reverse: 5′-CGG AAG ACC CCC TTC AAC | |||
| ECR 1 | CCCCACCCT | 223 | Forward: 5′-CTT GGT GGC CTG AGA AAA GG |
| Reverse: 5′-ACA GGC TCC AGC TAG GTC AC | |||
| ECR 2 | AGGGAGAGT | 205 | Forward: 5′-CAT GCC AGG GAG CTG TGA |
| Reverse: 5′-AGC TCT GCC CAA CAC ATG G | |||
| ECR 3 (1&2)* | 1-TCCCACCCC | 140 | Forward: 5′-AGG GAG ATC AGA CCG CTG AG |
| 2-GCCCACCCA | Reverse: 5′-GAG GCA GCT TCT CCA GGA AC | ||
| ECR 3 (3)* | AGGGTGGGG | 185 | Forward: 5′-ACT CGG ACT CCC CAG CTC |
| Reverse: 5′-GAA GGA GGG GAG CTG GTC | |||
| ECR 3 (4)* | GCCCGCCCT | 103 | Forward: 5′-CGC CAC TAT CAG TTG TTG G |
| Reverse: 5′-CAG TCC CCT GCT GCT CTG | |||
| ECR 6 | CCCCTCCCC | 179 | Forward: 5′-CCC TTC CTG TCT CCT CCA GT |
| Reverse: 5′-CTA TTG GGG CAA AGC TAG GA | |||
| ECR 7 | AGGGTGGGG | 169 | Forward: 5′-CTT GGT CTT CCA GAG GGA TG |
| Reverse: 5′-AGC CTT GGA GGA GGA GCT A | |||
| ECR 8 (1)* | CCACGCCCT | 169 | Forward: 5′-GGA GAG GAG ATG CGC TGA |
| Reverse: 5′-CTA TGA GGC AGG GGA AGA GA | |||
| ECR 8 (2)* | CCCCTCCCT | 182 | Forward: 5′-GCA CTC CTG TAG CCT ATC CTG |
| Reverse: 5′-TGC CAA GAC AAC CTC CTG | |||
| ECR 10 | AGGGTGGGG | 196 | Forward: 5′-GTC CAG GCT CAA CCT TTC C |
| Reverse: 5′-TGG GCT TCG TTC TCC ATA AA | |||
| ECR 12 (1)* | CCCCTCCCTC | 144 | Forward: 5′-GCT GGA GAC AGC TTC AAA GG |
| Reverse: 5′-GCA AGA AAG GAA ACA GTG AGG | |||
| ECR 12 (2&3)* | 2-CCCCTCCCT | 162 | Forward: 5′-CCT CCG ATT ATT GCA GGA |
| 3-GCCCACCCC | Reverse: 5′-CCG AGA AAG GCC TGA GAA G | ||
| ECR 12 (4)* | CGGGAGGGT | 141 | Forward: 5′-CCA TCA CAA AGA GCC TTA ATC C |
| Reverse: 5′-GGG TGT TGC CCC AGT TTT A |
Target region . | Central CACC site sequence . | Product size, bp, or reference . | Primer sequences . |
|---|---|---|---|
| β-Major globin | CCACACCCT | Letting et al37 | Forward: 5′-GAC AAA CAT TAT TCA GAG GGA GTA CCC |
| Reverse: 5′-AGG TGC ACC ATG ATG TAT GTT TAT GG | |||
| Nectin | N/A | Letting et al37 | Forward: 5′-TTT ACA TAA GCC TAG TGG TAC CCT |
| Reverse: 5′-ATC GCT GTC CTG CAT CTC ACA GTC G | |||
| ECR a | TGGGTGCGGC | 139 | Forward: 5′-AAT GAG AGG GTG GGT GGT TT |
| Reverse: 5′-CGG AAG ACC CCC TTC AAC | |||
| ECR 1 | CCCCACCCT | 223 | Forward: 5′-CTT GGT GGC CTG AGA AAA GG |
| Reverse: 5′-ACA GGC TCC AGC TAG GTC AC | |||
| ECR 2 | AGGGAGAGT | 205 | Forward: 5′-CAT GCC AGG GAG CTG TGA |
| Reverse: 5′-AGC TCT GCC CAA CAC ATG G | |||
| ECR 3 (1&2)* | 1-TCCCACCCC | 140 | Forward: 5′-AGG GAG ATC AGA CCG CTG AG |
| 2-GCCCACCCA | Reverse: 5′-GAG GCA GCT TCT CCA GGA AC | ||
| ECR 3 (3)* | AGGGTGGGG | 185 | Forward: 5′-ACT CGG ACT CCC CAG CTC |
| Reverse: 5′-GAA GGA GGG GAG CTG GTC | |||
| ECR 3 (4)* | GCCCGCCCT | 103 | Forward: 5′-CGC CAC TAT CAG TTG TTG G |
| Reverse: 5′-CAG TCC CCT GCT GCT CTG | |||
| ECR 6 | CCCCTCCCC | 179 | Forward: 5′-CCC TTC CTG TCT CCT CCA GT |
| Reverse: 5′-CTA TTG GGG CAA AGC TAG GA | |||
| ECR 7 | AGGGTGGGG | 169 | Forward: 5′-CTT GGT CTT CCA GAG GGA TG |
| Reverse: 5′-AGC CTT GGA GGA GGA GCT A | |||
| ECR 8 (1)* | CCACGCCCT | 169 | Forward: 5′-GGA GAG GAG ATG CGC TGA |
| Reverse: 5′-CTA TGA GGC AGG GGA AGA GA | |||
| ECR 8 (2)* | CCCCTCCCT | 182 | Forward: 5′-GCA CTC CTG TAG CCT ATC CTG |
| Reverse: 5′-TGC CAA GAC AAC CTC CTG | |||
| ECR 10 | AGGGTGGGG | 196 | Forward: 5′-GTC CAG GCT CAA CCT TTC C |
| Reverse: 5′-TGG GCT TCG TTC TCC ATA AA | |||
| ECR 12 (1)* | CCCCTCCCTC | 144 | Forward: 5′-GCT GGA GAC AGC TTC AAA GG |
| Reverse: 5′-GCA AGA AAG GAA ACA GTG AGG | |||
| ECR 12 (2&3)* | 2-CCCCTCCCT | 162 | Forward: 5′-CCT CCG ATT ATT GCA GGA |
| 3-GCCCACCCC | Reverse: 5′-CCG AGA AAG GCC TGA GAA G | ||
| ECR 12 (4)* | CGGGAGGGT | 141 | Forward: 5′-CCA TCA CAA AGA GCC TTA ATC C |
| Reverse: 5′-GGG TGT TGC CCC AGT TTT A |
Parenthetic values indicate CACC sites within the ECR