Table 2.

Gene-corrected (GFP+) peripheral blood cells in all 4 dogs 8 weeks following intravenous gene therapy as determined by flow cytometry

Neutrophils
Monocytes
Lymphocytes
CD3+
CD21+
Dog no.
No.*
% GFP+
No. GFP+*
No.*
% GFP+
No. GFP+*
No.*
% GFP+
No. GFP+*
%
No.*
% GFP+
No. GFP+*
%
No.*
% GFP+
No. GFP+*
1551   5 420   1.0   54   340   0.6   2   560   38.7   216   49.1   274   71.1   195   28.7   160   8.4   14  
1548   11 000   3.2   352   770   5.9   45   7200   52.8   3802   64.8   4665   78.5   3662   7.9   568   26.5   151  
1547   5 900   1.5   88   860   4.5   39   3400   65.2   2217   69.5   2363   78.0   1843   4.9   167   6.1   10  
1655
 		 6 320
 		 3.8
 		 242
 		 930
 		 11.1
 		 102
 		 3850
 		 61.0
 		 2348
 		 65.3
 		 2514
 		 84.5
 		 2120
 		 20.3
 		 782
 		 24.2
 		 189
 
Neutrophils
Monocytes
Lymphocytes
CD3+
CD21+
Dog no.
No.*
% GFP+
No. GFP+*
No.*
% GFP+
No. GFP+*
No.*
% GFP+
No. GFP+*
%
No.*
% GFP+
No. GFP+*
%
No.*
% GFP+
No. GFP+*
1551   5 420   1.0   54   340   0.6   2   560   38.7   216   49.1   274   71.1   195   28.7   160   8.4   14  
1548   11 000   3.2   352   770   5.9   45   7200   52.8   3802   64.8   4665   78.5   3662   7.9   568   26.5   151  
1547   5 900   1.5   88   860   4.5   39   3400   65.2   2217   69.5   2363   78.0   1843   4.9   167   6.1   10  
1655
 		 6 320
 		 3.8
 		 242
 		 930
 		 11.1
 		 102
 		 3850
 		 61.0
 		 2348
 		 65.3
 		 2514
 		 84.5
 		 2120
 		 20.3
 		 782
 		 24.2
 		 189
 

Normal ranges were determined in 8-week-old unaffected dogs and are as follows: neutrophils, 8500 ± 2900 cells/μL; monocytes, 1400 ± 700 cells/μL; lymphocytes, 5000 ± 1500 cells/μL; CD3+, 76.2% ± 14.4%; and CD21+, 12.9% ± 8.6%. The percentage of GFP+ cells in untreated control dogs (background signal) was less than 0.05%.

To convert neutrophil, monocyte, or lymphocyte counts to × 109 cells/L, divide cells/μL by 1000.

*

Cells/μL.

View Large
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal