Amplification of SRC clones in mice that underwent transplantation with CD34+ cells after SFSD culture
. | No. clones (no. mice) . | Fold amplification of clones, no. of clones (%) . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | |||||
| Experiment 1 | 20 (10) | 14 (70) | 3 (15) | 0 (0) | 1 (5) | 1 (5) | 1 (5) | |||||
| Experiment 2 | 15 (6) | 12 (80) | 3 (20) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | |||||
| Experiment 3 | 39 (10) | 28 (71.8) | 6 (15.4) | 3 (7.7) | 2 (5.1) | 0 (0) | 0 (0) | |||||
| Total | 74 | 54 | 24 | 9 | 12 | 5 | 6 | |||||
. | No. clones (no. mice) . | Fold amplification of clones, no. of clones (%) . | . | . | . | . | . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
. | . | 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | |||||
| Experiment 1 | 20 (10) | 14 (70) | 3 (15) | 0 (0) | 1 (5) | 1 (5) | 1 (5) | |||||
| Experiment 2 | 15 (6) | 12 (80) | 3 (20) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | |||||
| Experiment 3 | 39 (10) | 28 (71.8) | 6 (15.4) | 3 (7.7) | 2 (5.1) | 0 (0) | 0 (0) | |||||
| Total | 74 | 54 | 24 | 9 | 12 | 5 | 6 | |||||
SFSD-cultured CD34+ cells were divided into 10 groups, and transplanted directly into the bone marrow of 10 mice. In experiment 2, 4 mice died before examination. If the same clone was engrafted in 3 mice, it was assumed that the clone was amplified 3-fold by SFSD culture.