Luminescence intensity of human AChE-R, RACK1, and PKCϵ in Mks
Antibody . | FVBN . | TgR . | P . |
|---|---|---|---|
| None | 116.8 ± 8.7 | 122.4 ± 11.8 | NS |
| Hu AChE-R | 130.94 ± 18.26 | 212.3 ± 15.0 | < .001 |
| RACK1 | 153.4 ± 21.0 | 162.3 ± 49.2 | NS |
| PKCϵ | 160.9 ± 19.7 | 187.8 ± 22.7 | < .001 |
Antibody . | FVBN . | TgR . | P . |
|---|---|---|---|
| None | 116.8 ± 8.7 | 122.4 ± 11.8 | NS |
| Hu AChE-R | 130.94 ± 18.26 | 212.3 ± 15.0 | < .001 |
| RACK1 | 153.4 ± 21.0 | 162.3 ± 49.2 | NS |
| PKCϵ | 160.9 ± 19.7 | 187.8 ± 22.7 | < .001 |
Luminescence levels (from 1, low luminescence, to 220, bright) plus or minus SD were determined using an upright Zeiss microscope, ImagePro image capture, and Adobe Photoshop V 5.5 analysis for each megakaryocyte stained in the bone marrow smears (n = 50 per antibody).
NS indicates not significant using Student t test. Background staining was detected by incubation with no primary antibody.