Differentially expressed genes in memory and naive CD8+ T cells*
Gene name . | Gene symbol . | Accession no. . | Function . | Group† . |
|---|---|---|---|---|
| Memory cells highly expressed genes | ||||
| Integrin, beta 1 | ITGB1 | NM_002211 | Cell adhesion and signal transduction | M-1 |
| Proline-serine-threonine phosphatase interacting protein 1 | PSTPIP1 | NM_003978 | Cell adhesion and signal transduction | M-2 |
| Epstein-Barr virus induced gene 2 | EBI2 | NM_004951 | G-protein coupled receptor signaling | M-1 |
| Platelet activating receptor homolog | GPR171 | AF002986 | G-protein coupled receptor signaling | M-1 |
| G protein-coupled receptor 65 | GPR65 | BC035633 | G-protein coupled receptor signaling | M-2 |
| Arachidonate 5-lipoxygenase-activating protein | ALOX5AP | NM_001629 | Immune response | M-1 |
| Chemokine (C-C motif) receptor 5 | CCR5 | NM_000579 | Immune response | M-1 |
| Chemokine (C-X-C motif) receptor 6 | CXCR6 | NM_006564 | Immune response | M-1 |
| Granzyme K | GZMK | NM_002104 | Immune response | M-1 |
| Killer cell lectin-like receptor subfamily B-1 | KLRB1 | NM_002258 | Immune response | M-1 |
| Interferon-induced protein 44 | IFI44 | NM_006417 | Immune response | M-2 |
| Interleukin 18 receptor 1 | IL18R1 | NM_003855 | Immune response | M-2 |
| Interleukin 18 receptor accessory protein | IL18RAP | NM_003853 | Immune response | M-2 |
| S100 calcium binding protein A11 (calgizzarin) | S100A11 | NM_005620 | Regulation of cell proliferation | M-1 |
| Signaling lymphocytic activation molecule family 1 | SLAMF1 | NM_003037 | Regulation of cell proliferation | M-1 |
| CCAAT/enhancer binding protein delta | CEBPD | NM_005195 | Regulation of transcription | M-1 |
| Sin3-associated polypeptide | SAP30 | NM_003864 | Regulation of transcription | M-1 |
| Basic helix-loop-helix domain containing class B-2 | BHLHB2 | NM_003670 | Regulation of transcription | M-2 |
| Tumor necrosis factor, alpha-induced protein 3 | TNFAIP3 | NM_006290 | Regulation of transcription | M-2 |
| cAMP responsive element modulator | CREM | NM_183013 | Regulation of transcription | M-2 |
| Regulator of G-protein signaling 1 | RGS1 | NM_002922 | Signal transduction | M-1 |
| Dual specificity phosphatase 10 | DUSP10 | NM_007207 | Signal transduction | M-2 |
| EphA4 | EPHA4 | NM_004438 | Signal transduction | M-2 |
| Protein kinase C and casein kinase substrate in neurons 1 | PACSIN1 | NM_020804 | Signal transduction | M-2 |
| Regulator of G-protein signaling 2, 24 kDa | RGS2 | NM_002923 | Signal transduction | M-2 |
| T-cell receptor interacting molecule | TRIM | NM_016388 | Signal transduction | M-1 |
| Interleukin 4 induced 1 | IL4I1 | NM_172374 | Structure and metabolism | M-1 |
| Lymphocyte-activation gene 3 | LAG3 | NM_002286 | Structure and metabolism | M-1 |
| Sialyltransferase 8A | SIAT8A | NM_003034 | Structure and metabolism | M-1 |
| DnaJ (Hsp40) homolog, subfamily C, member 1 | DNAJC1 | NM_022365 | Structure and metabolism | M-2 |
| Villin 2 (ezrin) | Ezrin | AF188896 | Structure and metabolism | M-2 |
| Wiskott-Aldrich syndrome protein interacting protein | WASPIP | NM_003387 | Structure and metabolism | M-2 |
| Naive cells highly expressed genes | ||||
| Nerve growth factor receptor associated protein 1 | NGFRAP1 | NM_014380 | Immune response | N-1 |
| Fc fragment of IgG binding protein | FCGBP | NM_003890 | Immune response | N-1 |
| Adenylate kinase 5 | AK5 | BC012467 | Signal transduction | N-1 |
| Lymphoid enhancer-binding factor 1 | LEF1 | NM_016269 | Signal transduction | N-1 |
| Proprotein convertase subtilisin/kexin type 5 | PCSK5 | NM_006200 | Signal transduction | N-1 |
| Mal, T-cell differentiation protein | MAL | NM_002371 | Signal transduction | N-2 |
| SNF-1 related kinase | SNRK | NM_017719 | Signal transduction | N-2 |
| HMT1 hnRNP methyltransferase-like 1 | HRMTIL1 | NM_001535 | Signal transduction | N-2 |
| Actinin, alpha 1 | ACTN1 | NM_001102 | Structure and metabolism | N-1 |
| Keratin protein K6irs | K6IRS2 | NM_080747 | Structure and metabolism | N-1 |
| Kelch-like 3 (Drosophila) | KLHL3 | NM_017415 | Structure and metabolism | N-1 |
| S100 calcium binding protein, beta (neural) | S100B | NM_006272 | Structure and metabolism | N-2 |
Gene name . | Gene symbol . | Accession no. . | Function . | Group† . |
|---|---|---|---|---|
| Memory cells highly expressed genes | ||||
| Integrin, beta 1 | ITGB1 | NM_002211 | Cell adhesion and signal transduction | M-1 |
| Proline-serine-threonine phosphatase interacting protein 1 | PSTPIP1 | NM_003978 | Cell adhesion and signal transduction | M-2 |
| Epstein-Barr virus induced gene 2 | EBI2 | NM_004951 | G-protein coupled receptor signaling | M-1 |
| Platelet activating receptor homolog | GPR171 | AF002986 | G-protein coupled receptor signaling | M-1 |
| G protein-coupled receptor 65 | GPR65 | BC035633 | G-protein coupled receptor signaling | M-2 |
| Arachidonate 5-lipoxygenase-activating protein | ALOX5AP | NM_001629 | Immune response | M-1 |
| Chemokine (C-C motif) receptor 5 | CCR5 | NM_000579 | Immune response | M-1 |
| Chemokine (C-X-C motif) receptor 6 | CXCR6 | NM_006564 | Immune response | M-1 |
| Granzyme K | GZMK | NM_002104 | Immune response | M-1 |
| Killer cell lectin-like receptor subfamily B-1 | KLRB1 | NM_002258 | Immune response | M-1 |
| Interferon-induced protein 44 | IFI44 | NM_006417 | Immune response | M-2 |
| Interleukin 18 receptor 1 | IL18R1 | NM_003855 | Immune response | M-2 |
| Interleukin 18 receptor accessory protein | IL18RAP | NM_003853 | Immune response | M-2 |
| S100 calcium binding protein A11 (calgizzarin) | S100A11 | NM_005620 | Regulation of cell proliferation | M-1 |
| Signaling lymphocytic activation molecule family 1 | SLAMF1 | NM_003037 | Regulation of cell proliferation | M-1 |
| CCAAT/enhancer binding protein delta | CEBPD | NM_005195 | Regulation of transcription | M-1 |
| Sin3-associated polypeptide | SAP30 | NM_003864 | Regulation of transcription | M-1 |
| Basic helix-loop-helix domain containing class B-2 | BHLHB2 | NM_003670 | Regulation of transcription | M-2 |
| Tumor necrosis factor, alpha-induced protein 3 | TNFAIP3 | NM_006290 | Regulation of transcription | M-2 |
| cAMP responsive element modulator | CREM | NM_183013 | Regulation of transcription | M-2 |
| Regulator of G-protein signaling 1 | RGS1 | NM_002922 | Signal transduction | M-1 |
| Dual specificity phosphatase 10 | DUSP10 | NM_007207 | Signal transduction | M-2 |
| EphA4 | EPHA4 | NM_004438 | Signal transduction | M-2 |
| Protein kinase C and casein kinase substrate in neurons 1 | PACSIN1 | NM_020804 | Signal transduction | M-2 |
| Regulator of G-protein signaling 2, 24 kDa | RGS2 | NM_002923 | Signal transduction | M-2 |
| T-cell receptor interacting molecule | TRIM | NM_016388 | Signal transduction | M-1 |
| Interleukin 4 induced 1 | IL4I1 | NM_172374 | Structure and metabolism | M-1 |
| Lymphocyte-activation gene 3 | LAG3 | NM_002286 | Structure and metabolism | M-1 |
| Sialyltransferase 8A | SIAT8A | NM_003034 | Structure and metabolism | M-1 |
| DnaJ (Hsp40) homolog, subfamily C, member 1 | DNAJC1 | NM_022365 | Structure and metabolism | M-2 |
| Villin 2 (ezrin) | Ezrin | AF188896 | Structure and metabolism | M-2 |
| Wiskott-Aldrich syndrome protein interacting protein | WASPIP | NM_003387 | Structure and metabolism | M-2 |
| Naive cells highly expressed genes | ||||
| Nerve growth factor receptor associated protein 1 | NGFRAP1 | NM_014380 | Immune response | N-1 |
| Fc fragment of IgG binding protein | FCGBP | NM_003890 | Immune response | N-1 |
| Adenylate kinase 5 | AK5 | BC012467 | Signal transduction | N-1 |
| Lymphoid enhancer-binding factor 1 | LEF1 | NM_016269 | Signal transduction | N-1 |
| Proprotein convertase subtilisin/kexin type 5 | PCSK5 | NM_006200 | Signal transduction | N-1 |
| Mal, T-cell differentiation protein | MAL | NM_002371 | Signal transduction | N-2 |
| SNF-1 related kinase | SNRK | NM_017719 | Signal transduction | N-2 |
| HMT1 hnRNP methyltransferase-like 1 | HRMTIL1 | NM_001535 | Signal transduction | N-2 |
| Actinin, alpha 1 | ACTN1 | NM_001102 | Structure and metabolism | N-1 |
| Keratin protein K6irs | K6IRS2 | NM_080747 | Structure and metabolism | N-1 |
| Kelch-like 3 (Drosophila) | KLHL3 | NM_017415 | Structure and metabolism | N-1 |
| S100 calcium binding protein, beta (neural) | S100B | NM_006272 | Structure and metabolism | N-2 |
Selected differentially expressed genes in memory and naive CD8+ T cells are presented (the complete list of genes and transcripts can be found in Table S3).
Based on the expression changes after activation, genes are divided into 2 groups: (1) differences are maintained at 16 and 72 hours designated as M-1 or N-1, and (2) differences are decreased at 16 and 72 hours designated as M-2 and N-2, where M indicates memory cell and N indicates naive cell.