CFH characteristics and properties
. | ααHb . | PBH . | MP4 . |
|---|---|---|---|
| Hb, g/dL | 4.1 | 13 | 4.2 |
| MetHb, % | < 5 | < 5 | < 5 |
| MW, kDa (source) | 67.6 (Vandegriff et al23 ) | 180 (product insert)† | 96 (Vandegriff et al10 ) |
| Molecular radius, nm (source) | 3.1 (Vandegriff et al23 ) | 4.5 (Vandegriff et al23 ) | 9.3 (Friedemann et al19 ) |
| Molecular volume, nm3 | 127 | 382 | 3368 |
| COP, mmHg | 14 | 39 | 50 (Vandegriff et al10 ) |
| Viscosity, cp | 0.9 | 1.8 | 2.2 (Vandegriff et al10 ) |
| p50, mmHg | 23 (Vandegriff et al23 ) | 38 (Light et al24 ) | 5-6 (Vandegriff et al10 ) |
| NO binding rate,* μM–1s–1 | 31 (Rohlfs et al2 ) | 24 (Sakai‡) | 23 (Vandegriff et al3 ) |
. | ααHb . | PBH . | MP4 . |
|---|---|---|---|
| Hb, g/dL | 4.1 | 13 | 4.2 |
| MetHb, % | < 5 | < 5 | < 5 |
| MW, kDa (source) | 67.6 (Vandegriff et al23 ) | 180 (product insert)† | 96 (Vandegriff et al10 ) |
| Molecular radius, nm (source) | 3.1 (Vandegriff et al23 ) | 4.5 (Vandegriff et al23 ) | 9.3 (Friedemann et al19 ) |
| Molecular volume, nm3 | 127 | 382 | 3368 |
| COP, mmHg | 14 | 39 | 50 (Vandegriff et al10 ) |
| Viscosity, cp | 0.9 | 1.8 | 2.2 (Vandegriff et al10 ) |
| p50, mmHg | 23 (Vandegriff et al23 ) | 38 (Light et al24 ) | 5-6 (Vandegriff et al10 ) |
| NO binding rate,* μM–1s–1 | 31 (Rohlfs et al2 ) | 24 (Sakai‡) | 23 (Vandegriff et al3 ) |
Hb indicates Hb concentration; metHb, metHb concentration; MW, molecular weight; Molecular radius, indirectly determined from colloid osmotic pressure; Molecular volume, calculated from the molecular radius assuming a spherical shape; COP, colloid osmotic pressure measured at 25°C viscosity (Colloid Osmometer, 4100; Wescor, Logan UT); Viscosity, 37°C shear rate at 160–1 (DV-II+; Brookfield, Middleboro, MA); and p50, the partial pressure of oxygen at which the hemoglobin is 50% saturated
NO binding rates measured by rapid mixing stopped-flow apparatus
Average (range, 32-500 kDA). Distribution of hemoglobin polymers with less than 5% of the hemoglobin as unstabilized tetramers, approximately 50% has a molecular weight between 65 and 130 kDa, and no more than 10% has a molecular weight more than 500 kDa
Personal communication, Dr. Hiromi Sakai, Waseda University, Tokyo, Japan, e-mail April 12, 2006