Primers and probes used to discriminate the different 5′ GR-promoter and 3′ GR transcripts (GR-alpha, GR-beta, and GR-P) expression in pediatric ALL
GR-1A1 | |
| Forward | 5′-CAC TGG ACC TTA GAA GTT GAT A-3′ |
| Reverse | 5′-ATA CAG TCC CAT TGA GAG TGA-3′ |
| Probe | 5′-(FAM)-CCC TAA GAG GAG GAG CTA CTG AA-(TAMRA)-3′ |
| GR-1A2 | |
| Forward | 5′-GAA TAG AAA CAG AAA GAG GTT GAT A-3′ |
| Reverse | 5′-ATA CAG TCC CAT TGA GAG TGA-3′ |
| Probe | 5′-(FAM)-CCC TAA GAG GAG GAG CTA CTG AA-(TAMRA)-3′ |
| GR-1A3 | |
| Forward | 5′-AGT GTC TGA GAA GGA AGT TGA TA-3′ |
| Reverse | 5′-ATA CAG TCC CAT TGA GAG TGA-3′ |
| Probe | 5′-(FAM)-CCC TAA GAG GAG GAG CTA CTG AA-(TAMRA)-3′ |
| GR-1B | |
| Forward | 5′-GGC CCA AAT TGA TAT TCA-3′ |
| Reverse | 5′-ATA CAG TCC CAT TGA GAG TGA-3′ |
| Probe | 5′-(FAM)-CCC TAA GAG GAG GAG CTA CTG AA-(TAMRA)-3′ |
| GR-1C | |
| Forward | 5′-CTG CTC CTT CTG CGT TC-3′ |
| Reverse | 5′-ATA CAG TCC CAT TGA GAG TGA-3′ |
| Probe | 5′-(FAM)-CCC TAA GAG GAG GAG CTA CTG AA-(TAMRA)-3′ |
| GR-alpha | |
| Forward | 5′-TGT TTT GCT CCT GAT CTG A-3′ |
| Reverse | 5′-TCG GGG AAT TCA ATA CTC A-3′ |
| Probe | 5′-(FAM)-TGA CTC TAC CCT GCA TGT ACG AC-(TAMRA)-3′ |
| GR-beta | |
| Forward | 5′-TGT TTT GCT CCT GAT CTG A-3′ |
| Reverse | 5′-TGA GCG CCA AGA TTG T-3′ |
| Probe | 5′-(FAM)-TGA CTC TAC CCT GCA TGT ACG AC-(TAMRA)-3′ |
| GR-P | |
| Forward | 5′-TGT TTT GCT CCT GAT CTG A-3′ |
| Reverse | 5′-CCT TTG TTT CTA GGC CTT C-3′ |
| Probe | 5′-(FAM)-TGA CTC TAC CCT GCA TGT ACG AC-(TAMRA)-3′ |
| GAPDH | |
| Forward | 5′-GTC GGA GTC AAC GGA TT-3′ |
| Reverse | 5′-AAGCTT CCC GTT CTC AG-3′ |
| Probe | 5′-FAM-TCA ACT ACA TGG TTT ACA TGT TCC AA-(TAMRA)-3′ |
| RNaseP | |
| Forward | 5′-TTG GGA AGG TCT GAG ACT A-3′ |
| Reverse | 5′-TCA GCC ATT GAA CTC ACT T-3′ |
| Probe | 5′-(FAM)-AGG TCA GAC TGG GCA GGA GAT-(TAMRA)-3′ |
GR-1A1 | |
| Forward | 5′-CAC TGG ACC TTA GAA GTT GAT A-3′ |
| Reverse | 5′-ATA CAG TCC CAT TGA GAG TGA-3′ |
| Probe | 5′-(FAM)-CCC TAA GAG GAG GAG CTA CTG AA-(TAMRA)-3′ |
| GR-1A2 | |
| Forward | 5′-GAA TAG AAA CAG AAA GAG GTT GAT A-3′ |
| Reverse | 5′-ATA CAG TCC CAT TGA GAG TGA-3′ |
| Probe | 5′-(FAM)-CCC TAA GAG GAG GAG CTA CTG AA-(TAMRA)-3′ |
| GR-1A3 | |
| Forward | 5′-AGT GTC TGA GAA GGA AGT TGA TA-3′ |
| Reverse | 5′-ATA CAG TCC CAT TGA GAG TGA-3′ |
| Probe | 5′-(FAM)-CCC TAA GAG GAG GAG CTA CTG AA-(TAMRA)-3′ |
| GR-1B | |
| Forward | 5′-GGC CCA AAT TGA TAT TCA-3′ |
| Reverse | 5′-ATA CAG TCC CAT TGA GAG TGA-3′ |
| Probe | 5′-(FAM)-CCC TAA GAG GAG GAG CTA CTG AA-(TAMRA)-3′ |
| GR-1C | |
| Forward | 5′-CTG CTC CTT CTG CGT TC-3′ |
| Reverse | 5′-ATA CAG TCC CAT TGA GAG TGA-3′ |
| Probe | 5′-(FAM)-CCC TAA GAG GAG GAG CTA CTG AA-(TAMRA)-3′ |
| GR-alpha | |
| Forward | 5′-TGT TTT GCT CCT GAT CTG A-3′ |
| Reverse | 5′-TCG GGG AAT TCA ATA CTC A-3′ |
| Probe | 5′-(FAM)-TGA CTC TAC CCT GCA TGT ACG AC-(TAMRA)-3′ |
| GR-beta | |
| Forward | 5′-TGT TTT GCT CCT GAT CTG A-3′ |
| Reverse | 5′-TGA GCG CCA AGA TTG T-3′ |
| Probe | 5′-(FAM)-TGA CTC TAC CCT GCA TGT ACG AC-(TAMRA)-3′ |
| GR-P | |
| Forward | 5′-TGT TTT GCT CCT GAT CTG A-3′ |
| Reverse | 5′-CCT TTG TTT CTA GGC CTT C-3′ |
| Probe | 5′-(FAM)-TGA CTC TAC CCT GCA TGT ACG AC-(TAMRA)-3′ |
| GAPDH | |
| Forward | 5′-GTC GGA GTC AAC GGA TT-3′ |
| Reverse | 5′-AAGCTT CCC GTT CTC AG-3′ |
| Probe | 5′-FAM-TCA ACT ACA TGG TTT ACA TGT TCC AA-(TAMRA)-3′ |
| RNaseP | |
| Forward | 5′-TTG GGA AGG TCT GAG ACT A-3′ |
| Reverse | 5′-TCA GCC ATT GAA CTC ACT T-3′ |
| Probe | 5′-(FAM)-AGG TCA GAC TGG GCA GGA GAT-(TAMRA)-3′ |