Details of extraction procedures and MS methods used
Before sample treatment . | Crude membrane digest . |
|---|---|
| EtOH treatment: FT-Q-STAR | EtOH-treated crude membrane analyzed by LTQ-FT (1st peptide count) versus Q-STAR (2nd peptide count) |
| Carbonate and EtOH:sat-100 mM | Crude membrane treated with carbonate solution followed by EtOH: saturated carbonate solution versus 100 mM |
| Carbonate × 2:sat-100 mM | Crude membrane treated twice with carbonate: saturated solution versus 100 mM |
| Carbonate × 2 and EtOH:sat-100 mM | Crude membrane treated twice with carbonate solution followed by EtOH: saturated carbonate solution versus 100 mM |
| Gels: FT-Q-STAR | Gel analyzed with FT versus Q-STAR |
| NO_Cytosk: normal-low threshold | Cytoskeletal removal on crude membranes analyzed by normal versus low threshold |
| Exclusion lists of_NO_Cytosk | Exclusion list of the above samples |
| NO_Cytosk + EtOH | Cytoskeletal removal on crude membranes followed by EtOH treatment |
| Exclusions_NO_Cytosk + EtOH: 1 × -2 × | Exclusion list of the above samples run once versus run twice |
Before sample treatment . | Crude membrane digest . |
|---|---|
| EtOH treatment: FT-Q-STAR | EtOH-treated crude membrane analyzed by LTQ-FT (1st peptide count) versus Q-STAR (2nd peptide count) |
| Carbonate and EtOH:sat-100 mM | Crude membrane treated with carbonate solution followed by EtOH: saturated carbonate solution versus 100 mM |
| Carbonate × 2:sat-100 mM | Crude membrane treated twice with carbonate: saturated solution versus 100 mM |
| Carbonate × 2 and EtOH:sat-100 mM | Crude membrane treated twice with carbonate solution followed by EtOH: saturated carbonate solution versus 100 mM |
| Gels: FT-Q-STAR | Gel analyzed with FT versus Q-STAR |
| NO_Cytosk: normal-low threshold | Cytoskeletal removal on crude membranes analyzed by normal versus low threshold |
| Exclusion lists of_NO_Cytosk | Exclusion list of the above samples |
| NO_Cytosk + EtOH | Cytoskeletal removal on crude membranes followed by EtOH treatment |
| Exclusions_NO_Cytosk + EtOH: 1 × -2 × | Exclusion list of the above samples run once versus run twice |
The table lists and explains the headings of Figure 1, detailing the extraction procedures and MS methods used. The first number in each column always refers to the amount of peptides detected with the first method and is compared with the second number, which is the amount of peptides detected with the second method.