SDF-1α levels in cocultures of BM stroma and BCCs
Cells . | Coculture . | SDF-1α, pg/mL . |
|---|---|---|
| BC cell lines | - | 80 ± 8 |
| Primary BCCs | - | 56 ± 6 |
| BM stroma | - | 22 ± 4 |
| BCCs + stroma | ||
| 4 cell lines | + | 10 ± 3* |
| 4 primary cells | + | 15 ± 3* |
Cells . | Coculture . | SDF-1α, pg/mL . |
|---|---|---|
| BC cell lines | - | 80 ± 8 |
| Primary BCCs | - | 56 ± 6 |
| BM stroma | - | 22 ± 4 |
| BCCs + stroma | ||
| 4 cell lines | + | 10 ± 3* |
| 4 primary cells | + | 15 ± 3* |
BC cell lines (n = 4: T47D, DU4475, BT 483, ZR-75-30) and primary BCCs (P1, P2, P4) were established as cocultures with BM stroma or cultured alone as described.21 Each BCC was studied with stroma from a different donor. At 80% confluence, culture media were collected and then studied for levels of SDF-1α using ELISA. The results are presented as mean ± SD; n = 7.
- indicates cells cultured alone; +, cocultures.
P < .5 versus BCCs (cell lines and primary cells) cultured alone.