Alloengraftment in sensitized mice
Treatment . | MFI,* 4-6 wk . | Engraftment . |
---|---|---|
None | 227.6 ± 74.8 | 0 of 6 |
CyP | 165.4 ± 58.3 | 1 of 7 |
Anti-CD8 + CyP | 379.9 ± 187.1 | 2 of 6 |
Anti-αβ-TCR | 314.7 ± 174.9 | 0 of 7 |
Anti-αβ-TCR + CyP | 326.0 ± 150.0 | 1/9 |
Anti-αβ-TCR/CD8 | 282.6 ± 52.8 | 0 of 3 |
Anti-αβ-TCR/CD8 + CyP | 229.1 ± 56.3 | 0 of 8 |
Anti-CD8/CD154 | 196.4 ± 134.7 | 1 of 12 |
Splenocytes + CyP + anti-CD8/CD154 + CyP | 360.7 ± 224.2 | 1 of 8 |
Anti-αβ-TCR/class I + CyP | 225.9 ± 108.1 | 0 of 4 |
Anti-MHC recipient/class II | 229.7 ± 152.0 | 0 of 3 |
Anti-NK1.1 | 278.4 ± 125.5 | 0 of 7 |
Anti-CD8/CD154/NK1.1 | 212.7 ± 102.4 | 0 of 7 |
Anti-CD45RB | 306.1 ± 124.4 | 0 of 6 |
Anti-CD8/CD45RB | 245.8 ± 121.2 | 0 of 7 |
Cobra venom factor (CVF) | 405.3 ± 101.0 | 0 of 4 |
CVF + CyA | 269.0 ± 151.9 | 0 of 4 |
CVF + CyA + anti-CD154 | 256.6 ± 154.2 | 0 of 6 |
Anti-CD8/CD154 + fludarabine | 201.7 ± 72.9 | 0 of 4 |
Anti-CD8/CD154 + rapamycin | 208.3 ± 63.5 | 0 of 4 |
Anti-CD8/CD154 + rapamycin + CyA | 356.7 ± 127.6 | 0 of 4 |
MMF | 203.8 ± 96.2 | 0 of 4 |
Anti-CD8/CD154 + MMF | 195.9 ± 127.0 | 1 of 8 |
Rapamycin + CyA | 200.6 ± 114.2 | 0 of 6 |
Rapamycin + CyA + splenectomy | 306.7 ± 169.9 | 0 of 4 |
Rapamycin + CyA + anti-CD154 | 369.7 ± 190.6 | 0 of 4 |
Anti-B220 | 371.6 ± 155.3 | 0 of 6 |
3 × 80 × 106 BMCs | 192.3 ± 155.5 | 0 of 10 |
Subtractive immunization | 298.3 ± 125.7 | 0 of 8 |
Treatment . | MFI,* 4-6 wk . | Engraftment . |
---|---|---|
None | 227.6 ± 74.8 | 0 of 6 |
CyP | 165.4 ± 58.3 | 1 of 7 |
Anti-CD8 + CyP | 379.9 ± 187.1 | 2 of 6 |
Anti-αβ-TCR | 314.7 ± 174.9 | 0 of 7 |
Anti-αβ-TCR + CyP | 326.0 ± 150.0 | 1/9 |
Anti-αβ-TCR/CD8 | 282.6 ± 52.8 | 0 of 3 |
Anti-αβ-TCR/CD8 + CyP | 229.1 ± 56.3 | 0 of 8 |
Anti-CD8/CD154 | 196.4 ± 134.7 | 1 of 12 |
Splenocytes + CyP + anti-CD8/CD154 + CyP | 360.7 ± 224.2 | 1 of 8 |
Anti-αβ-TCR/class I + CyP | 225.9 ± 108.1 | 0 of 4 |
Anti-MHC recipient/class II | 229.7 ± 152.0 | 0 of 3 |
Anti-NK1.1 | 278.4 ± 125.5 | 0 of 7 |
Anti-CD8/CD154/NK1.1 | 212.7 ± 102.4 | 0 of 7 |
Anti-CD45RB | 306.1 ± 124.4 | 0 of 6 |
Anti-CD8/CD45RB | 245.8 ± 121.2 | 0 of 7 |
Cobra venom factor (CVF) | 405.3 ± 101.0 | 0 of 4 |
CVF + CyA | 269.0 ± 151.9 | 0 of 4 |
CVF + CyA + anti-CD154 | 256.6 ± 154.2 | 0 of 6 |
Anti-CD8/CD154 + fludarabine | 201.7 ± 72.9 | 0 of 4 |
Anti-CD8/CD154 + rapamycin | 208.3 ± 63.5 | 0 of 4 |
Anti-CD8/CD154 + rapamycin + CyA | 356.7 ± 127.6 | 0 of 4 |
MMF | 203.8 ± 96.2 | 0 of 4 |
Anti-CD8/CD154 + MMF | 195.9 ± 127.0 | 1 of 8 |
Rapamycin + CyA | 200.6 ± 114.2 | 0 of 6 |
Rapamycin + CyA + splenectomy | 306.7 ± 169.9 | 0 of 4 |
Rapamycin + CyA + anti-CD154 | 369.7 ± 190.6 | 0 of 4 |
Anti-B220 | 371.6 ± 155.3 | 0 of 6 |
3 × 80 × 106 BMCs | 192.3 ± 155.5 | 0 of 10 |
Subtractive immunization | 298.3 ± 125.7 | 0 of 8 |
Treatment consisted of the following: CyP, 100 mg/kg intraperitoneally at day 2; anti-αβTCR, 100 μg/each intraperitoneally at day –3; anti-CD8, 100 μg/each intraperitoneally at day –3; anti-CD154, 500 μg/each at days 0 and 3; anti-B220, 10 mg/each (SAS precipitated), intraperitoneally at days –21, –19, –17, –10, and –3; anti-class I, 400 μg/each intraperitoneally at day –1; anti-class II, 400 μg/each intraperitoneally at days –21, –19, –17, –10, and –3; anti-NK1.1, 1 mg/each intraperitoneally at days –3 and –1; splenectomy, at day –3; CD45RB, 1 mg (unpurified) intraperitoneally at days 0 and 1; CVF, 60 U/kg intraperitoneally at day –1 and 20 U/kg intraperitoneally at days 0, 1, 2, 3, 4, and 7; CyA, 15 mg/kg per day intraperitoneally at days 0 to 7; fludarabine, 100 mg/kg per day intraperitoneally at days –5 to –3; MMF, 20 mg/kg per day intraperitoneally at days –1 to 14; rapamycin, 3 mg/kg per day intraperitoneally at days 0 to 7; BMCs, 80 × 106 per day at days 0, 2, and 4; targeting B cells, CyP at days –30 and –15; IVIG, 1 mg/kg per day at days 1, 2, and 3; and subtractive immunization with 15 × 106 donor splenocytes at day –7, followed by CyP at days –6 and –5. MFI is presented as mean ± SD