Table 1.

Alloengraftment in sensitized mice


Treatment

MFI,* 4-6 wk

Engraftment
None   227.6 ± 74.8   0 of 6  
CyP   165.4 ± 58.3   1 of 7  
Anti-CD8 + CyP   379.9 ± 187.1   2 of 6  
Anti-αβ-TCR   314.7 ± 174.9   0 of 7  
Anti-αβ-TCR + CyP   326.0 ± 150.0   1/9  
Anti-αβ-TCR/CD8   282.6 ± 52.8   0 of 3  
Anti-αβ-TCR/CD8 + CyP   229.1 ± 56.3   0 of 8  
Anti-CD8/CD154   196.4 ± 134.7   1 of 12  
Splenocytes + CyP + anti-CD8/CD154 + CyP   360.7 ± 224.2   1 of 8  
Anti-αβ-TCR/class I + CyP   225.9 ± 108.1   0 of 4  
Anti-MHC recipient/class II   229.7 ± 152.0   0 of 3  
Anti-NK1.1   278.4 ± 125.5   0 of 7  
Anti-CD8/CD154/NK1.1   212.7 ± 102.4   0 of 7  
Anti-CD45RB   306.1 ± 124.4   0 of 6  
Anti-CD8/CD45RB   245.8 ± 121.2   0 of 7  
Cobra venom factor (CVF)   405.3 ± 101.0   0 of 4  
CVF + CyA   269.0 ± 151.9   0 of 4  
CVF + CyA + anti-CD154   256.6 ± 154.2   0 of 6  
Anti-CD8/CD154 + fludarabine   201.7 ± 72.9   0 of 4  
Anti-CD8/CD154 + rapamycin   208.3 ± 63.5   0 of 4  
Anti-CD8/CD154 + rapamycin + CyA   356.7 ± 127.6   0 of 4  
MMF   203.8 ± 96.2   0 of 4  
Anti-CD8/CD154 + MMF   195.9 ± 127.0   1 of 8  
Rapamycin + CyA   200.6 ± 114.2   0 of 6  
Rapamycin + CyA + splenectomy   306.7 ± 169.9   0 of 4  
Rapamycin + CyA + anti-CD154   369.7 ± 190.6   0 of 4  
Anti-B220   371.6 ± 155.3   0 of 6  
3 × 80 × 106 BMCs   192.3 ± 155.5   0 of 10  
Subtractive immunization
 
298.3 ± 125.7
 
0 of 8
 

Treatment

MFI,* 4-6 wk

Engraftment
None   227.6 ± 74.8   0 of 6  
CyP   165.4 ± 58.3   1 of 7  
Anti-CD8 + CyP   379.9 ± 187.1   2 of 6  
Anti-αβ-TCR   314.7 ± 174.9   0 of 7  
Anti-αβ-TCR + CyP   326.0 ± 150.0   1/9  
Anti-αβ-TCR/CD8   282.6 ± 52.8   0 of 3  
Anti-αβ-TCR/CD8 + CyP   229.1 ± 56.3   0 of 8  
Anti-CD8/CD154   196.4 ± 134.7   1 of 12  
Splenocytes + CyP + anti-CD8/CD154 + CyP   360.7 ± 224.2   1 of 8  
Anti-αβ-TCR/class I + CyP   225.9 ± 108.1   0 of 4  
Anti-MHC recipient/class II   229.7 ± 152.0   0 of 3  
Anti-NK1.1   278.4 ± 125.5   0 of 7  
Anti-CD8/CD154/NK1.1   212.7 ± 102.4   0 of 7  
Anti-CD45RB   306.1 ± 124.4   0 of 6  
Anti-CD8/CD45RB   245.8 ± 121.2   0 of 7  
Cobra venom factor (CVF)   405.3 ± 101.0   0 of 4  
CVF + CyA   269.0 ± 151.9   0 of 4  
CVF + CyA + anti-CD154   256.6 ± 154.2   0 of 6  
Anti-CD8/CD154 + fludarabine   201.7 ± 72.9   0 of 4  
Anti-CD8/CD154 + rapamycin   208.3 ± 63.5   0 of 4  
Anti-CD8/CD154 + rapamycin + CyA   356.7 ± 127.6   0 of 4  
MMF   203.8 ± 96.2   0 of 4  
Anti-CD8/CD154 + MMF   195.9 ± 127.0   1 of 8  
Rapamycin + CyA   200.6 ± 114.2   0 of 6  
Rapamycin + CyA + splenectomy   306.7 ± 169.9   0 of 4  
Rapamycin + CyA + anti-CD154   369.7 ± 190.6   0 of 4  
Anti-B220   371.6 ± 155.3   0 of 6  
3 × 80 × 106 BMCs   192.3 ± 155.5   0 of 10  
Subtractive immunization
 
298.3 ± 125.7
 
0 of 8
 

Treatment consisted of the following: CyP, 100 mg/kg intraperitoneally at day 2; anti-αβTCR, 100 μg/each intraperitoneally at day –3; anti-CD8, 100 μg/each intraperitoneally at day –3; anti-CD154, 500 μg/each at days 0 and 3; anti-B220, 10 mg/each (SAS precipitated), intraperitoneally at days –21, –19, –17, –10, and –3; anti-class I, 400 μg/each intraperitoneally at day –1; anti-class II, 400 μg/each intraperitoneally at days –21, –19, –17, –10, and –3; anti-NK1.1, 1 mg/each intraperitoneally at days –3 and –1; splenectomy, at day –3; CD45RB, 1 mg (unpurified) intraperitoneally at days 0 and 1; CVF, 60 U/kg intraperitoneally at day –1 and 20 U/kg intraperitoneally at days 0, 1, 2, 3, 4, and 7; CyA, 15 mg/kg per day intraperitoneally at days 0 to 7; fludarabine, 100 mg/kg per day intraperitoneally at days –5 to –3; MMF, 20 mg/kg per day intraperitoneally at days –1 to 14; rapamycin, 3 mg/kg per day intraperitoneally at days 0 to 7; BMCs, 80 × 106 per day at days 0, 2, and 4; targeting B cells, CyP at days –30 and –15; IVIG, 1 mg/kg per day at days 1, 2, and 3; and subtractive immunization with 15 × 106 donor splenocytes at day –7, followed by CyP at days –6 and –5. MFI is presented as mean ± SD

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