Identification of IL-5–producing CD4+ T cells in liver tissue of S mansoni–infected wild-type and eosinophil lineage–ablated mice
. | %lymphocytes . | %CD4+ . | %IL-5+ CD4+ . | IL-5+ CD4+/total CD4+ cells . |
|---|---|---|---|---|
| BALB/c | 0.13 | |||
| Experiment no. 1 | 17.9 | 5.5 | 0.73 | |
| Experiment no. 2 | 15.7 | 4.8 | 0.63 | |
| Average | 16.8 | 5.2 | 0.68 | |
| ΔdbIGATA | 0.11 | |||
| Experiment no. 1 | 38.3 | 13.9 | 1.6 | |
| Experiment no. 2 | 38.6 | 12.6 | 1.4 | |
| Average | 38.5 | 13.3 | 1.5 | |
| C57BL/6 | 0.32 | |||
| Experiment no. 1 | 38.7 | 11.8 | 3.9 | |
| Experiment no. 2 | 36.3 | 8.6 | 2.6 | |
| Average | 37.5 | 10.2 | 3.3 | |
| TgPHIL | 0.32 | |||
| Experiment no. 1 | 23.9 | 7.5 | 2.5 | |
| Experiment no. 2 | 26.8 | 6.9 | 2.1 | |
| Average | 25.4 | 7.2 | 2.3 |
. | %lymphocytes . | %CD4+ . | %IL-5+ CD4+ . | IL-5+ CD4+/total CD4+ cells . |
|---|---|---|---|---|
| BALB/c | 0.13 | |||
| Experiment no. 1 | 17.9 | 5.5 | 0.73 | |
| Experiment no. 2 | 15.7 | 4.8 | 0.63 | |
| Average | 16.8 | 5.2 | 0.68 | |
| ΔdbIGATA | 0.11 | |||
| Experiment no. 1 | 38.3 | 13.9 | 1.6 | |
| Experiment no. 2 | 38.6 | 12.6 | 1.4 | |
| Average | 38.5 | 13.3 | 1.5 | |
| C57BL/6 | 0.32 | |||
| Experiment no. 1 | 38.7 | 11.8 | 3.9 | |
| Experiment no. 2 | 36.3 | 8.6 | 2.6 | |
| Average | 37.5 | 10.2 | 3.3 | |
| TgPHIL | 0.32 | |||
| Experiment no. 1 | 23.9 | 7.5 | 2.5 | |
| Experiment no. 2 | 26.8 | 6.9 | 2.1 | |
| Average | 25.4 | 7.2 | 2.3 |
CD4+ T lymphocytes producing IL-5 were identified from single-cell suspensions from liver tissue by flow cytometric analysis as described in “Materials and methods.” Values shown represent percentages of total viable leukocytes isolated analyzed in 2 separate experiments of 3 to 5 mice each.