Secondary transplantation of BM reconstituted with SP- or MP-CD34-KSL cells
. | Primary Tx . | Secondary Tx . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Cell type . | Total . | Total . | Myeloid . | B lymphoid . | T lymphoid . | |||
| SP-CD34-KSL | ||||||||
| Recipient 1 | 64.1 | 13.4 ± 4.3 | 2.8 ± 1.6 | 10.2 ± 5.0 | 29.7 ± 9.6 | |||
| Recipient 2 | 53.2 | 59.1 ± 11.6 | 54.5 ± 21.5 | 87.1 ± 6.89 | 48.8 ± 19.1 | |||
| MP-CD34-KSL | ||||||||
| Recipient 1 | 55.9 | 27.6 ± 14.3 | 2.4 ± 2.0 | 40.6 ± 24.2 | 40.4 ± 19.8 | |||
| Recipient 2 | 50.5 | 6.1 ± 1.7 | 0.9 ± 0.9 | 3.8 ± 4.8 | 6.2 ± 2.6 | |||
. | Primary Tx . | Secondary Tx . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Cell type . | Total . | Total . | Myeloid . | B lymphoid . | T lymphoid . | |||
| SP-CD34-KSL | ||||||||
| Recipient 1 | 64.1 | 13.4 ± 4.3 | 2.8 ± 1.6 | 10.2 ± 5.0 | 29.7 ± 9.6 | |||
| Recipient 2 | 53.2 | 59.1 ± 11.6 | 54.5 ± 21.5 | 87.1 ± 6.89 | 48.8 ± 19.1 | |||
| MP-CD34-KSL | ||||||||
| Recipient 1 | 55.9 | 27.6 ± 14.3 | 2.4 ± 2.0 | 40.6 ± 24.2 | 40.4 ± 19.8 | |||
| Recipient 2 | 50.5 | 6.1 ± 1.7 | 0.9 ± 0.9 | 3.8 ± 4.8 | 6.2 ± 2.6 | |||
Four months after primary transplantation, 2 × 106 BM cells from 2 recipient mice reconstituted with 30 SP-CD34-KSL cells and from 2 recipient mice reconstituted with 30 MP-CD34-KSL cells (Table 1) were transplanted into irradiated mice
Secondary recipient mice were analyzed 4 months after transplantation. The percent chimerism is shown as mean ± SD for total leukocytes, myeloid cells, B-lymphoid cells, and T-lymphoid cells. For the secondary recipients of the first mouse of each cell type, n = 9; for those of the second, n = 10
Tx indicates transplantation