Single-cell reconstitution with SP- or MP-CD34-KSL cells
. | . | Percent chimerism . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Cell type . | LTR (%) . | Total . | Myeloid . | B-lymphoid . | T-lymphoid . | |||
| CD34 KSL | 10/44 (22.7) | 21.6 ± 20.3 | 38.0 ± 34.0 | 14.6 ± 15.3 | 10.8 ± 8.6 | |||
| SP-CD34-KSL | 11/52 (21.2) | 26.7 ± 24.8 | 41.1 ± 31.4 | 23.5 ± 28.2 | 14.5 ± 23.2 | |||
| MP-CD34-KSL | 7/41 (17.1) | 25.1 ± 16.4 | 38.2 ± 26.9 | 18.3 ± 20.2 | 14.7 ± 16.5 | |||
. | . | Percent chimerism . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
| Cell type . | LTR (%) . | Total . | Myeloid . | B-lymphoid . | T-lymphoid . | |||
| CD34 KSL | 10/44 (22.7) | 21.6 ± 20.3 | 38.0 ± 34.0 | 14.6 ± 15.3 | 10.8 ± 8.6 | |||
| SP-CD34-KSL | 11/52 (21.2) | 26.7 ± 24.8 | 41.1 ± 31.4 | 23.5 ± 28.2 | 14.5 ± 23.2 | |||
| MP-CD34-KSL | 7/41 (17.1) | 25.1 ± 16.4 | 38.2 ± 26.9 | 18.3 ± 20.2 | 14.7 ± 16.5 | |||
Single CD34-KSL cells, SP-CD34-KSL cells, or MP-CD34-KSL cells were transplanted into lethally irradiated mice, together with 2 × 105 competitor cells. Peripheral blood cells of the recipient mice were analyzed 4 months after transplantation
Long-term reconstitution (LTR) was considered to have occurred when total chimerism was more than 1%. Data show the number of reconstituted mice per the number of mice that received transplants and the percent chimerism (of mice considered to have undergone long-term reconstitution) expressed as mean ± SD. The frequency of repopulating cells among MP-CD34-KSL cells does not differ from that among SP-CD34-KSL (P = .79, Fisher exact test)