Inhibition of erythroid expansion by infected erythrocytes (IE), IE lysate, TNF-α, uninfected erythrocytes (UEs), and UE lysate
Time of cell, cytokine, or lysate addition/day of measurement of growth inhibition . | IEs, % of control (SD) . | IE lysate, % of control (SD) . | TNF-α (30 ng/mL),*% of control (SD) . | UEs, % of control (SD) . | UE lysate,†% of control (SD) . |
|---|---|---|---|---|---|
| Day 0 | |||||
| Day 11 | 73.9 (12.6)‡ | 63.7 (26.3)§ | 78.2 (9.9)‡ | 82.2 (6.39) | 89.5 (7.21) |
| Day 14 | 67.3 (26.2)§ | 55.9 (30.9)§ | 71.5 (22.6)‡ | 90.6 (5.4) | 84.9 (1.9)‡ |
| Day 4* | |||||
| Day 11 | 45.4 (27.7)‡ | 55.6 (32.5)‡ | 74.8 (12.9)‡ | 90.4 (15.3) | 111.7 (22.0) |
| Day 14 | 61.1 (38.5)‡ | 58.5 (37.8)‡ | 93.2 (26.3) | 91.8 (9.3) | 110.9 (8.3) |
| Day 7† | |||||
| Day 11 | 79 (10.3) | 82 (0.9)|| | 80.3 (8.3)‡ | ND | ND |
| Day 14 | 92.6 (10.8) | 98.7 (5.07) | 98.5 (8.5) | ND | ND |
Time of cell, cytokine, or lysate addition/day of measurement of growth inhibition . | IEs, % of control (SD) . | IE lysate, % of control (SD) . | TNF-α (30 ng/mL),*% of control (SD) . | UEs, % of control (SD) . | UE lysate,†% of control (SD) . |
|---|---|---|---|---|---|
| Day 0 | |||||
| Day 11 | 73.9 (12.6)‡ | 63.7 (26.3)§ | 78.2 (9.9)‡ | 82.2 (6.39) | 89.5 (7.21) |
| Day 14 | 67.3 (26.2)§ | 55.9 (30.9)§ | 71.5 (22.6)‡ | 90.6 (5.4) | 84.9 (1.9)‡ |
| Day 4* | |||||
| Day 11 | 45.4 (27.7)‡ | 55.6 (32.5)‡ | 74.8 (12.9)‡ | 90.4 (15.3) | 111.7 (22.0) |
| Day 14 | 61.1 (38.5)‡ | 58.5 (37.8)‡ | 93.2 (26.3) | 91.8 (9.3) | 110.9 (8.3) |
| Day 7† | |||||
| Day 11 | 79 (10.3) | 82 (0.9)|| | 80.3 (8.3)‡ | ND | ND |
| Day 14 | 92.6 (10.8) | 98.7 (5.07) | 98.5 (8.5) | ND | ND |
Inhibition is expressed as percentage of expansion of control cultures after 11 and 14 days of culture without added medium, cells, or TNF-α
Uninfected red blood cells (UE), infected erythrocytes (IE), and their respective lysates were added at a ratio of 1 developing erythroid cell per 50 UEs or IEs or their lysates. The mean and SD were calculated from 7 independent experiments unless specified otherwise
ND indicates not done on day 7 cultures
N = 5
N = 3
P < .05 compared with control wells
P < .01 compared with control wells
P < .001 compared with control wells