Propagation of GFP-positive cells after adoptive transfer of cells isolated from intestine grafts into lethally irradiated WT recipients
Source and number of cells transferred . | No. of recipients . | Survival, d . | % GFP-positive cells in the blood, day 100 . |
|---|---|---|---|
| Without additional BM cells | |||
| None | 5 | 8, 9, 11, 11, 12 | NA |
| Naive GFP BM, 2 × 106 | 2 | > 100 | > 96 |
| Naive WT BM, 2 × 106 | 2 | > 100 | 0 |
| ACI host BM 100 d after transplantation of WT intestine and GFP BM, 2 × 106 | 3 | > 100 | 1.1 ± 0.8 |
| With 2 × 106 WT BM cells | |||
| Naive GFP MLNs and/or PPs, 10 × 106 | 4 | > 100 | 0.8 ± 0.9 |
| WT graft MLNs and/or PPs 100 d after transplantation of WT intestine and GFP BM, 8 × 106 to 12.4 × 106 | 3 | > 100 | 5.9 ± 0.4 |
Source and number of cells transferred . | No. of recipients . | Survival, d . | % GFP-positive cells in the blood, day 100 . |
|---|---|---|---|
| Without additional BM cells | |||
| None | 5 | 8, 9, 11, 11, 12 | NA |
| Naive GFP BM, 2 × 106 | 2 | > 100 | > 96 |
| Naive WT BM, 2 × 106 | 2 | > 100 | 0 |
| ACI host BM 100 d after transplantation of WT intestine and GFP BM, 2 × 106 | 3 | > 100 | 1.1 ± 0.8 |
| With 2 × 106 WT BM cells | |||
| Naive GFP MLNs and/or PPs, 10 × 106 | 4 | > 100 | 0.8 ± 0.9 |
| WT graft MLNs and/or PPs 100 d after transplantation of WT intestine and GFP BM, 8 × 106 to 12.4 × 106 | 3 | > 100 | 5.9 ± 0.4 |
Data represent mean ± SD.
NA indicates not applicable.