Peritoneal leukocyte differentials in thioglycollate-challenged mice
Time after injection, genotype . | P . | Total leukocytes (n) . | Macrophages, % (n) . | Granulocytes, % (n) . | Lymphocytes, % (n) . |
|---|---|---|---|---|---|
| 0 h | — | ||||
| Wild type | 5.1 ± 0.6 (5) | 70.2 ± 1.1 (5) | 26.5 ± 3.6 (5) | 1.1 ± 0.8 (5) | |
| Siat1ΔP1 | 5.0 ± 1.4 (5) | 71.0 ± 2.4 (3) | 24.1 ± 2.8 (3) | 3.9 ± 1.3 (3) | |
| 5 h | .002 | ||||
| Wild type | 6.0 ± 2.2 (8) | 32.6 ± 5.0 (4) | 65.3 ± 5.4 (4) | 0.8 ± 0.4 (4) | |
| Siat1ΔP1 | 11.4 ± 3.1 (8) | 27.2 ± 5.0 (4) | 70.7 ± 5.5 (4) | 1.0 ± 1.1 (4) | |
| 18 h | .002 | ||||
| Wild type | 18.6 ± 5.4 (10) | 39.7 ± 3.3 (8) | 59.1 ± 3.6 (8) | 0.8 ± 0.2 (8) | |
| Siat1ΔP1 | 30.0 ± 6.0 (10) | 33.1 ± 3.2 (8) | 65.7 ± 3.5 (8) | 1.0 ± 0.5 (8) | |
| 80 h | .014 | ||||
| Wild type | 41.4 ± 7.8 (8) | 88.5 ± 3.7 (5) | 9.9 ± 3.7 (5) | 1.6 ± 0.4 (5) | |
| Siat1ΔP1 | 51.0 ± 6.0 (8) | 89.2 ± 1.7 (5) | 9.6 ± 1.5 (5) | 1.2 ± 0.4 (5) |
Time after injection, genotype . | P . | Total leukocytes (n) . | Macrophages, % (n) . | Granulocytes, % (n) . | Lymphocytes, % (n) . |
|---|---|---|---|---|---|
| 0 h | — | ||||
| Wild type | 5.1 ± 0.6 (5) | 70.2 ± 1.1 (5) | 26.5 ± 3.6 (5) | 1.1 ± 0.8 (5) | |
| Siat1ΔP1 | 5.0 ± 1.4 (5) | 71.0 ± 2.4 (3) | 24.1 ± 2.8 (3) | 3.9 ± 1.3 (3) | |
| 5 h | .002 | ||||
| Wild type | 6.0 ± 2.2 (8) | 32.6 ± 5.0 (4) | 65.3 ± 5.4 (4) | 0.8 ± 0.4 (4) | |
| Siat1ΔP1 | 11.4 ± 3.1 (8) | 27.2 ± 5.0 (4) | 70.7 ± 5.5 (4) | 1.0 ± 1.1 (4) | |
| 18 h | .002 | ||||
| Wild type | 18.6 ± 5.4 (10) | 39.7 ± 3.3 (8) | 59.1 ± 3.6 (8) | 0.8 ± 0.2 (8) | |
| Siat1ΔP1 | 30.0 ± 6.0 (10) | 33.1 ± 3.2 (8) | 65.7 ± 3.5 (8) | 1.0 ± 0.5 (8) | |
| 80 h | .014 | ||||
| Wild type | 41.4 ± 7.8 (8) | 88.5 ± 3.7 (5) | 9.9 ± 3.7 (5) | 1.6 ± 0.4 (5) | |
| Siat1ΔP1 | 51.0 ± 6.0 (8) | 89.2 ± 1.7 (5) | 9.6 ± 1.5 (5) | 1.2 ± 0.4 (5) |
Peritoneal leukocytes were recovered by peritoneal lavage without further treatment (0 h) and at 5, 18, and 80 hours after intraperitoneal thioglycollate injection. Differential counts (expressed as percentage of total population) of leukocyte subsets determined by staining of exudate smears. Percentage of granulocytes is the total of eosinophils and neutrophils. Total leukocyte counts are given in million per animal.
n indicates number of mice used for each determination; —, not applicable.