Recovery of CFSE- and PKH-labeled donor cells in peritoneum of thioglycollate-elicited recipients
. | Donor CFSE/PKH . | Recovered CFSE/PKH . |
|---|---|---|
| ΔP1-CFSE/ΔP1-PKH donor | ||
| ΔP1 recipient 1 | 1.30 | 1.3 |
| ΔP1 recipient 2 | 1.30 | 1.3 |
| ΔP1-CFSE/WT-PKH donor | ||
| WT recipient 1 | 1.24 | 1.3 |
| WT recipient 2 | 1.24 | 1.2 |
| WT recipient 3 | 1.24 | 1.2 |
| WT recipient 4 | 1.24 | 1.0 |
| ΔP1 recipient 1 | 1.24 | 0.8 |
| ΔP1 recipient 2 | 1.24 | 1.2 |
| ΔP1 recipient 3 | 1.24 | 1.2 |
| ΔP1 recipient 4 | 1.24 | 1.2 |
. | Donor CFSE/PKH . | Recovered CFSE/PKH . |
|---|---|---|
| ΔP1-CFSE/ΔP1-PKH donor | ||
| ΔP1 recipient 1 | 1.30 | 1.3 |
| ΔP1 recipient 2 | 1.30 | 1.3 |
| ΔP1-CFSE/WT-PKH donor | ||
| WT recipient 1 | 1.24 | 1.3 |
| WT recipient 2 | 1.24 | 1.2 |
| WT recipient 3 | 1.24 | 1.2 |
| WT recipient 4 | 1.24 | 1.0 |
| ΔP1 recipient 1 | 1.24 | 0.8 |
| ΔP1 recipient 2 | 1.24 | 1.2 |
| ΔP1 recipient 3 | 1.24 | 1.2 |
| ΔP1 recipient 4 | 1.24 | 1.2 |
Total bone marrow cells from donors labeled with CFSE of PKH-26 were pooled and injected into tail veins of recipient animals. At the same time, 1 mL of 4% wt/vol thioglycollate was introduced intraperitoneally into recipients. After 6 hours, peritoneal aspirate was recovered from recipients and examined by flow cytometry.