Expression of cytochrome P450 (CYP450) and histamine receptor (HR) mRNA in K562 cells and KU812 cells
Gene product . | Product size, bp . | Expression of mRNA . | . | |
|---|---|---|---|---|
| . | . | K562 cells . | KU812 cells . | |
| CYP450 1A1 | 146 | + | + | |
| CYP450 1A2 | 226 | – | – | |
| CYP450 1B1 | 360 | – | – | |
| CYP450 2A6 | 589 | + | + | |
| CYP450 2A7 | 774 | + | + | |
| CYP450 2A13 | 320 | – | – | |
| CYP450 2C9 | 357 | – | – | |
| CYP450 2D6 | 419 | + | + | |
| CYP450 2E1 | 246 | + | – | |
| CYP450 3A4 | 353 | + | – | |
| CYP450 3A5 | 432 | + | + | |
| CYP450 3A7 | 447 | – | – | |
| HR-1 | 150 | + | + | |
| HR-2 | 128 | + | + | |
| HR-3 | 140 | – | – | |
| HR-4 | 200 | + | + | |
Gene product . | Product size, bp . | Expression of mRNA . | . | |
|---|---|---|---|---|
| . | . | K562 cells . | KU812 cells . | |
| CYP450 1A1 | 146 | + | + | |
| CYP450 1A2 | 226 | – | – | |
| CYP450 1B1 | 360 | – | – | |
| CYP450 2A6 | 589 | + | + | |
| CYP450 2A7 | 774 | + | + | |
| CYP450 2A13 | 320 | – | – | |
| CYP450 2C9 | 357 | – | – | |
| CYP450 2D6 | 419 | + | + | |
| CYP450 2E1 | 246 | + | – | |
| CYP450 3A4 | 353 | + | – | |
| CYP450 3A5 | 432 | + | + | |
| CYP450 3A7 | 447 | – | – | |
| HR-1 | 150 | + | + | |
| HR-2 | 128 | + | + | |
| HR-3 | 140 | – | – | |
| HR-4 | 200 | + | + | |
Expression of transcripts specific for cytochrome P450 (CYP450) isoenzymes and histamine receptors (HR) type 1-4 in K562 cells and KU812 cells was analyzed by RT-PCR using primers specific for CYP450 isoenzymes47 and histamine receptors