Effect of B-cell-derived IL-10 on GVHD
Graft . | TCD Allo . | Allo . | Allo . | Allo . |
|---|---|---|---|---|
| Recipient chimera | μMT → wt | μMT → wt | μMT + wt → wt | μMT + IL-10-/- → wt |
| μMT/wt or IL-10-/- BM ratio | 1:0 | 1:0 | 4:1 | 4:1 |
| Recipient B cell no. | 0.2 ± 0.1 | 0.2 ± 0.1 | 32 ± 8 | 21 ± 3 |
| B-cell IL-10 | NA | NA | 929 ± 83 | <30 |
| Survival, % | 90 | 29 | 65* | 33 |
Graft . | TCD Allo . | Allo . | Allo . | Allo . |
|---|---|---|---|---|
| Recipient chimera | μMT → wt | μMT → wt | μMT + wt → wt | μMT + IL-10-/- → wt |
| μMT/wt or IL-10-/- BM ratio | 1:0 | 1:0 | 4:1 | 4:1 |
| Recipient B cell no. | 0.2 ± 0.1 | 0.2 ± 0.1 | 32 ± 8 | 21 ± 3 |
| B-cell IL-10 | NA | NA | 929 ± 83 | <30 |
| Survival, % | 90 | 29 | 65* | 33 |
Wild-type (wt) CD45.1+ B6 mice were transplanted with CD45.2+ μMT or a combination of wild-type and μMT or IL-10-/- and μMT T-cell-depleted (TCD) BM in the ratios described and left for 4 months to reconstitute. At this time B-cell numbers were quantitated within the spleen (expressed as mean ± SE per spleen [×106]), and FACS-sorted B cells were stimulated in culture for 48 hours with LPS (10 μg/mL) and IL-10 determined in culture supernatant (pg/mL). Chimeras were subsequently transplanted with allogeneic TCD BM without (TCD Allo, n = 10) or with splenic T cells (Allo, n = 18-20 per group in 2 experiments) from Balb/c donors and survival determined at day 45 by Kaplan-Meier estimates.
NA indicates not applicable.
P < .05 versus μMT → wild type and μMT + IL-10-/- → wild type.