Absolute counts of CD3+CD4+ and CD3+CD8+ lymphocytes, B220+IgM+ B cells, and NK cells evaluated in the peripheral blood of ADA–/– mice 6 months after GT (ADA–/– GT) or BMT (ADA–/– BMT)
. | . | Mean no. cells × 106/mL ± SD . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
. | N . | CD3+CD4+ T cells . | CD3+CD8+ T cells . | B220+IgM+ B cells . | NK cells . | |||
| ADA-/- GT | 6 | 2.72 ± 1.14 | 1.13 ± 0.33 | 0.43 ± 0.25 | 0.33 ± 0.05 | |||
| ADA-/- BMT | 6 | 3.25 ± 1.39 | 1.33 ± 0.48 | 0.39 ± 0.19 | 0.51 ± 0.22 | |||
| ADA+/+ untreated | 6 | 2.24 ± 0.27 | 1.12 ± 0.22 | 0.26 ± 0.11 | 0.31 ± 0.07 | |||
| ADA-/- untreated* | 14 | 0.14 ± 0.16 | 0.11 ± 0.20 | 0.40 ± 0.25 | 0.19 ± 0.16 | |||
| ADA+/+ untreated* | 8 | 2.90 ± 0.70 | 1.00 ± 0.20 | 2.00 ± 0.40 | 0.90 ± 0.10 | |||
. | . | Mean no. cells × 106/mL ± SD . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|
. | N . | CD3+CD4+ T cells . | CD3+CD8+ T cells . | B220+IgM+ B cells . | NK cells . | |||
| ADA-/- GT | 6 | 2.72 ± 1.14 | 1.13 ± 0.33 | 0.43 ± 0.25 | 0.33 ± 0.05 | |||
| ADA-/- BMT | 6 | 3.25 ± 1.39 | 1.33 ± 0.48 | 0.39 ± 0.19 | 0.51 ± 0.22 | |||
| ADA+/+ untreated | 6 | 2.24 ± 0.27 | 1.12 ± 0.22 | 0.26 ± 0.11 | 0.31 ± 0.07 | |||
| ADA-/- untreated* | 14 | 0.14 ± 0.16 | 0.11 ± 0.20 | 0.40 ± 0.25 | 0.19 ± 0.16 | |||
| ADA+/+ untreated* | 8 | 2.90 ± 0.70 | 1.00 ± 0.20 | 2.00 ± 0.40 | 0.90 ± 0.10 | |||
No statistically significant differences were observed among ADA-/- GT, ADA-/- BMT, and adult untreated ADA+/+ mice (2-tailed Mann-Whitney test). At 18 days of age all subpopulations were significantly reduced in ADA-/- mice compared with age-matched controls (P < .01).
Eighteen-day-old ADA-/- or ADA+/+ mice are shown as controls.