Kinetics of neutrophil influx in popliteal lymph nodes
. | OVA/CFA . | . | PBS/CFA . | . | ||
|---|---|---|---|---|---|---|
| Time, h* . | OVA-FITC+ cells . | Neutrophil infiltrates . | OVA-FITC+ cells . | Neutrophil infiltrates . | ||
| 2 | 5 ± 1 | 8 ± 2 | 1 ± 1 | 2 ± 1 | ||
| 6 | 30 ± 2 | 39 ± 2 | 1 ± 1 | 2 ± 1 | ||
| 24 | ND | 15 ± 2 | ND | ND | ||
| 48 | ND | 2 ± 1 | ND | ND | ||
. | OVA/CFA . | . | PBS/CFA . | . | ||
|---|---|---|---|---|---|---|
| Time, h* . | OVA-FITC+ cells . | Neutrophil infiltrates . | OVA-FITC+ cells . | Neutrophil infiltrates . | ||
| 2 | 5 ± 1 | 8 ± 2 | 1 ± 1 | 2 ± 1 | ||
| 6 | 30 ± 2 | 39 ± 2 | 1 ± 1 | 2 ± 1 | ||
| 24 | ND | 15 ± 2 | ND | ND | ||
| 48 | ND | 2 ± 1 | ND | ND | ||
Kinetics of OVA-FITC+ cells or NE influx at longer time periods in LN sections evaluated by microscopic analysis (×400). The data are expressed as the number of cells per field. At least 20 and 40 fields of each smear were evaluated.
The results were not detected (ND) for 72 hours and 10 days.