Densitometry analysis of nuclear p52 and p100
| . | Medium . | rhBAFF . | rhBAFF + anti-BR3, 1 μg/mL . | rhBAFF + anti-BR3, 3 μg/mL . | rhBAFF + anti-BR3, 10 μg/mL . |
|---|---|---|---|---|---|
| p52 | 1.00 ± 0.00 | 47.41 ± 7.20 | 27.45 ± 0.72 | 9.29 ± 2.94 | 1.73 ± 0.57 |
| p100 | 1.00 ± 0.00 | 0.13 ± 0.07 | 0.18 ± 0.09 | 0.38 ± 0.30 | 1.04 ± 0.47 |
| . | Medium . | rhBAFF . | rhBAFF + anti-BR3, 1 μg/mL . | rhBAFF + anti-BR3, 3 μg/mL . | rhBAFF + anti-BR3, 10 μg/mL . |
|---|---|---|---|---|---|
| p52 | 1.00 ± 0.00 | 47.41 ± 7.20 | 27.45 ± 0.72 | 9.29 ± 2.94 | 1.73 ± 0.57 |
| p100 | 1.00 ± 0.00 | 0.13 ± 0.07 | 0.18 ± 0.09 | 0.38 ± 0.30 | 1.04 ± 0.47 |
CLL B cells were cultured with or without rhBAFF (50 ng/mL) at the indicated concentration of anti-BR3 antibody for immunoblot analysis. Defined sections of the immunoblot film were analyzed with image analysis software from the National Institutes of Health. Results are presented as density of the specific bands using extracts of treated cells relative to that of bands from extracts of CLL cells cultured in medium alone. Data are shown as mean ± SD from experiments performed on leukemia samples from 3 patients. Anti-BR3 antibody at 10 μg/mL could completely inhibit BAFF-induced translocation of p52.