Flow cytometric analysis of IL-15-pretreated IL-15–/– B6 BM cell donors
. | BM cell donor/pretreatment, percent bone marrow cells (P) . | . | . | ||
|---|---|---|---|---|---|
| Cell type . | WT B6/PBS . | IL-15-/- B6/PBS . | IL-15-/- B6/rhIL-15 . | ||
| Monocytes/granulocytes | 31.7 | 31.3 (.76) | 29.3 (.16) | ||
| Myeloid dendritic cells | 1.84 | 1.28 (.1) | 2.01 (.41) | ||
| Plasmacytoid dendritic cells | 2.72 | 2.26 (.009) | 2.63 (.74) | ||
| B lymphocytes | 22.3 | 25.9 (.12) | 18.6 (.04) | ||
| Natural killer cells | 1.47 | 0.16 (< .001) | 5.5 (< .001) | ||
. | BM cell donor/pretreatment, percent bone marrow cells (P) . | . | . | ||
|---|---|---|---|---|---|
| Cell type . | WT B6/PBS . | IL-15-/- B6/PBS . | IL-15-/- B6/rhIL-15 . | ||
| Monocytes/granulocytes | 31.7 | 31.3 (.76) | 29.3 (.16) | ||
| Myeloid dendritic cells | 1.84 | 1.28 (.1) | 2.01 (.41) | ||
| Plasmacytoid dendritic cells | 2.72 | 2.26 (.009) | 2.63 (.74) | ||
| B lymphocytes | 22.3 | 25.9 (.12) | 18.6 (.04) | ||
| Natural killer cells | 1.47 | 0.16 (< .001) | 5.5 (< .001) | ||
Donor mice were pretreated with rhIL-15 or PBS as described in “Results.” Values represent mean percent of bone marrow cells that were CD11b+GR-1+ monocytes/granulocytes, CD11c+B220+ plasmacytoid dendritic cells, CD11c+B220- myeloid dendritic cells, CD11c-B220+ B cells, or NK1.1+CD3- NK cells. P values (in parentheses) were calculated using the Student t test for comparisons between wt B6 and IL-15- or control-treated IL-15-/- B6 donors. N = 10 donors per group.