Table 1

Genes regulated after ectopic expression of THAP1 in endothelial cells

Unigene IDGene symbolGene name and descriptionFold changePreviously identified as E2F targetPreviously identified as Rb target
Genes down-regulated after ectopic expression of THAP1 in primary ECs 
    Cell-cycle control      
        Hs.374378 CKS1B* CDC28 protein kinase regulatory subunit 1B (CKS1) −2.19 Yes35  Yes31  
        Hs.520506 FBXO5* F-box protein 5 (APCcdh1 inhibitor Emil) −1.83 Yes32  Yes32  
        Hs.334562 CDC2* Cell division cycle 2, G1 to S and G2 to M −1.81 Yes33-36  Yes29-31,36  
        Hs.194698 CCNB2* Cyclin B2 −1.66 Yes34,36  Yes29-31,36  
        Hs.23960 CCNBI* Cyclin B1 −1.61 Yes34,36  Yes29-31,36  
    DNA replication/DNA repair      
        Hs.234896 GMNN* Geminin, DNA replication inhibitor −2.02 No Yes29  
        Hs.198363 MCM10 MCM10 minichromosome maintenance-deficient 10 (S cerevisiae) −2.01 No No 
        Hs.409065 FEN1* Flap structure-specific endonuclease 1 −2 Yes32-36  Yes29,31,32,36  
        Hs.202672 DNMT1* DNA (cytosine-5-)-methyltransferase 1 −2 Yes33  Yes29,31  
        Hs.5199 HSPC150* Similar to ubiquitin-conjugating enzyme Ubc13p (S cerevisiae) −1.92 Yes35,36  Yes36  
        Hs.383396 RRM1* Ribonucleotide reductase M1 polypeptide −1.79 Yes32,34-36  Yes29-32,36  
        Hs.438720 MCM7* MCM7 minichromosome maintenance deficient 7 (S cerevisiae) −1.75 Yes32-34  Yes29-32  
        Hs.489037 ASK* Activator of S phase kinase −1.74 Yes35  Yes31  
        Hs.433180 PSF2 DNA replication complex GINS protein PSF2 −1.73 No No 
        Hs.226390 RRM2* Ribonucleotide reductase M2 polypeptide −1.58 Yes32,34  Yes29-32  
    Chromosome condensation and segregation      
        Hs.99819 USP16 Ubiquitin-specific protease 16 (associated with mitotic chromosomes) −3.37 No No 
        Hs.497741 CENPF* Centromere protein F, 350/400 kDa (mitosin) −2.8 Yes36  Yes31,36  
        Hs.75573 CENPE* Centromere protein E, 312 kDa −2.15 Yes35,36  Yes31,36  
        Hs.434953 HMGB2* High-mobility group box 2 (associated with mitotic chromosomes) −2.09 Yes32-34  Yes29-32  
        Hs.42650 ZWINT* Kinetochore protein ZW10 interactor −1.78 No Yes31  
        Hs.14559 C10orf3 Chromosome 10 open reading frame 3 (SMC-domain protein) −1.72 No No 
        Hs.5719 CNAP1 Chromosome condensation-related SMC-associated protein 1 −1.65 No No 
        Hs.350966 PTTG1* Securin (pituitary tumor-transforming 1) −1.63 Yes34-36  Yes29,31,36  
        Hs.521097 PTTG3 Pituitary tumor-transforming 3 −1.62 No No 
        Hs.511755 PTTG2 Pituitary tumor-transforming 2 −1.52 No No 
    Mitotic spindle checkpoint      
        Hs.421956 SPC25* Kinetochore protein Spc25 homologue (S cerevisiae) −2.04 Yes36  Yes29,31,36  
        Hs.234545 CDCA1 Kinetochore protein Nuf2 (cell division cycle-associated 1) −1.91 No No 
        Hs.533185 MAD2L1* Mitotic spindle assembly checkpoint protein MAD2 (MAD2-like 1) −1.91 Yes35,36  Yes31,36  
        Hs.36708 BUB1B* Mitotic checkpoint serine/threonine-protein kinase BUBR1 −1.85 Yes33,34,36  Yes29,31,36  
        Hs.414407 KNTC2* Kinetochore protein Hec1 (kinetochore-associated 2) −1.79 Yes35,36  Yes31,36  
        Hs.469649 BUB1* Mitotic checkpoint serine/threonine-protein kinase BUB1 −1.76 No Yes29-31  
        Hs.524947 CDC20* CDC20 cell division cycle 20 homologue (S cerevisiae) −1.6 Yes34  Yes29-31  
    Mitotic spindle assembly      
        Hs.121028 ASPM Abnormal spindle (asp)-like, microcephaly associated (Drosophila) −2.05 No No 
        Hs.514033 SPAG5 Mitotic spindle coiled-coil-related protein SPAG5 (MAP126) −1.91 No No 
        Hs.77695 DLG7* HURP/KIAA0008, discs large homologue 7 (Drosophila) −1.9 No Yes29,31  
        Hs.104019 TACC3 Transforming, acidic coiled-coil-containing protein 3 −1.8 No No 
        Hs.119324 KIF22* Kinesin family member 22 (KNSL4, kinesin-like 4) −1.76 Yes35,36  Yes29,31,36  
        Hs.90073 CSEIL* CSE1 chromosome segregation 1–like (yeast) −1.63 No Yes31  
        Hs.514527 BIRC5* Survivin (baculoviral IAP repeat-containing 5) −1.62 Yes36  Yes31,36  
        Hs.72550 HMMR* Hyaluronan-mediated motility receptor (RHAMM) −1.6 Yes35  Yes29,31  
        Hs.73625 KIF20A* Kinesin family member 20A (RAB6KIFL) −1.59 Yes36  Yes29,30,36  
        Hs.532793 KPNB1* Karyopherin (importin) beta 1 −1.58 Yes36  Yes36  
        Hs.69360 KIF2C* Kinesin family member 2C (KNSL6, kinesin-like 6) −1.53 Yes36  Yes31,36  
    Cytokinesis      
        Hs.126774 RAMP RA-regulated nuclear matrix-associated protein −1.69 No No 
        Hs.459362 PRCI* Protein regulator of cytokinesis 1 −1.66 Yes35,36  Yes29,36  
        Hs.62180 ANLN* Anillin, actin binding protein (scraps homologue, Drosophila) −1.61 Yes35,36  Yes29,36  
    Cell cycle/cell proliferation miscellaneous      
        Hs.470654 CDCA7 Cell division cycle-associated 7 (c-myc target gene JPO1) −2.55 No No 
        Hs.104741 PBK PDZ binding kinase (mitotic kinase TOPK) −1.89 No No 
        Hs.434886 CDCA5 Cell division cycle-associated 5 −1.88 No No 
        Hs.436187 TRIP13* Thyroid hormone receptor interactor 13 −1.78 No Yes30,31  
        Hs.239 FOXM1* Forkhead box M1 −1.65 No Yes31  
        Hs.89497 LMNB1* Lamin B1 −1.65 Yes33  Yes29-31  
        Hs.484813 DEK* DEK oncogene (DNA binding) −1.61 Yes32  Yes32  
        Hs.208912 C22orf18 Proliferation-associated nuclear element (PANE1) −1.56 No Yes29,36  
    Miscellaneous      
        Hs.421907 GLTSCR2 Glioma tumor suppressor candidate region gene 2 −4.41 No No 
        Hs.528306 U1SNRNPBP U11/U12 snRNP 35K −3.77 No No 
        Hs.497636 LAMB3 Laminin, beta 3 −2.91 No No 
        Hs.160411 TSHR Thyroid-stimulating hormone receptor −2.55 No No 
        Hs.7432 THAP1 THAP domain-containing, apoptosis-associated protein 1 −2.13 No No 
        Hs.248941 TAF9 TAF9 RNA polymerase II −2.08 No No 
        Hs.472054 C20orf42 Chromosome 20 open reading frame 42 (kindlerin KIND1) −1.86 No No 
        Hs.71827 RRS1 RRS1 ribosome biogenesis regulator homologue (S cerevisiae) −1.84 No No 
        Hs.296310 GJA4 Gap junction protein, alpha 4, 37kDa (connexin 37) −1.68 No No 
        Hs.472119 MKKS McKusick-Kaufman syndrome −1.62 No No 
        Hs.166539 ITGB3BP Integrin beta 3-binding protein (beta3-endonexin, NRIF3) −1.55 No No 
    Predicted proteins with unknown functions      
        Hs.327252 LZIC Leucine zipper and CTNNB1P1 domain containing −2.18 No No 
        Hs.388087 ZCSL2 Zinc finger, CSL domain-containing 2 −2.07 No No 
        Hs.525764 ARHGAP11A Rho GTPase activating protein 11A −2.03 No No 
        Hs.529778 FKSG14* Leucine zipper protein FKSG14 −2.01 Yes32  Yes32  
        Hs.508292 FLJ10514 Hypothetical protein FLJ10514 −1.89 No No 
        Hs.404323 FLJ10156 Hypothetical protein FLJ10156 −1.81 No No 
        Hs.121536 FAM54A Family with sequence similarity 54, member A −1.79 No No 
        Hs.162717 MGC15668 Hypothetical protein MGC15668 −1.7 No No 
        Hs.330663 FLJ20641 Hypothetical protein FLJ20641 −1.66 No No 
        Hs.127767 LOC130502 Similar to CG14894-PA −1.63 No No 
        Hs.426696 FLJ20516 Timeless-interacting protein −1.62 No No 
        Hs.209715 FLJ22624 FLJ22624 protein −1.6 No No 
        Hs.283532 BM039 Uncharacterized bone marrow protein BM039 −1.58 No No 
        Hs.550564 RPL7L1 Ribosomal protein L7-like 1 −1.53 No No 
        Hs.30696 TCFL5 Transcription factor-like 5 (basic helix-loop-helix) −1.5 No No 
Genes up-regulated after ectopic expression of THAP1 in primary EC 
    Miscellaneous      
        Hs.531081 LGALS3 Lectin, galactoside-binding, soluble, 3 (galectin 3) 1.52 No No 
        Hs.198308 WRB Tryptophan-rich basic protein 1.58 No No 
        Hs.387794 CHGN Chondroitin beta 1,4 N-acetylgalactosaminyltransferase 1.6 No No 
        Hs.45140 TMEM35 Transmembrane protein 35 1.65 No No 
        Hs.505141 FLJ14337 Similar to peptidylprolyl isomerase A, isoform 1 1.67 No No 
        Hs.549129 RP11-529110.4 Deleted in a mouse model of primary ciliary dyskinesia 1.69 No No 
        Hs.508835 TEP1 Telomerase-associated protein 1 1.7 No No 
        Hs.518731 UCHL1 Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) 1.71 No No 
        Hs.106511 PCDH17 Protocadherin 17 1.71 No No 
        Hs.492261 TP53INP1 Tumor protein p53 inducible nuclear protein 1 1.89 No No 
        Hs.126598 TMEM45A Transmembrane protein 45A 1.96 No No 
        Hs.391561 FABP4 Fatty acid binding protein 4, adipocyte 1.98 No No 
        Hs.386470 NMB Neuromedin B 2.01 No No 
        Hs.517581 HMOX1 Heme oxygenase (decycling) 1 3.06 No No 
        Hs.289292 FOXL2 Forkhead box L2 4.21 No No 
        Hs.546296 SECTM1 Secreted and transmembrane 1 5.51 No No 
Unigene IDGene symbolGene name and descriptionFold changePreviously identified as E2F targetPreviously identified as Rb target
Genes down-regulated after ectopic expression of THAP1 in primary ECs 
    Cell-cycle control      
        Hs.374378 CKS1B* CDC28 protein kinase regulatory subunit 1B (CKS1) −2.19 Yes35  Yes31  
        Hs.520506 FBXO5* F-box protein 5 (APCcdh1 inhibitor Emil) −1.83 Yes32  Yes32  
        Hs.334562 CDC2* Cell division cycle 2, G1 to S and G2 to M −1.81 Yes33-36  Yes29-31,36  
        Hs.194698 CCNB2* Cyclin B2 −1.66 Yes34,36  Yes29-31,36  
        Hs.23960 CCNBI* Cyclin B1 −1.61 Yes34,36  Yes29-31,36  
    DNA replication/DNA repair      
        Hs.234896 GMNN* Geminin, DNA replication inhibitor −2.02 No Yes29  
        Hs.198363 MCM10 MCM10 minichromosome maintenance-deficient 10 (S cerevisiae) −2.01 No No 
        Hs.409065 FEN1* Flap structure-specific endonuclease 1 −2 Yes32-36  Yes29,31,32,36  
        Hs.202672 DNMT1* DNA (cytosine-5-)-methyltransferase 1 −2 Yes33  Yes29,31  
        Hs.5199 HSPC150* Similar to ubiquitin-conjugating enzyme Ubc13p (S cerevisiae) −1.92 Yes35,36  Yes36  
        Hs.383396 RRM1* Ribonucleotide reductase M1 polypeptide −1.79 Yes32,34-36  Yes29-32,36  
        Hs.438720 MCM7* MCM7 minichromosome maintenance deficient 7 (S cerevisiae) −1.75 Yes32-34  Yes29-32  
        Hs.489037 ASK* Activator of S phase kinase −1.74 Yes35  Yes31  
        Hs.433180 PSF2 DNA replication complex GINS protein PSF2 −1.73 No No 
        Hs.226390 RRM2* Ribonucleotide reductase M2 polypeptide −1.58 Yes32,34  Yes29-32  
    Chromosome condensation and segregation      
        Hs.99819 USP16 Ubiquitin-specific protease 16 (associated with mitotic chromosomes) −3.37 No No 
        Hs.497741 CENPF* Centromere protein F, 350/400 kDa (mitosin) −2.8 Yes36  Yes31,36  
        Hs.75573 CENPE* Centromere protein E, 312 kDa −2.15 Yes35,36  Yes31,36  
        Hs.434953 HMGB2* High-mobility group box 2 (associated with mitotic chromosomes) −2.09 Yes32-34  Yes29-32  
        Hs.42650 ZWINT* Kinetochore protein ZW10 interactor −1.78 No Yes31  
        Hs.14559 C10orf3 Chromosome 10 open reading frame 3 (SMC-domain protein) −1.72 No No 
        Hs.5719 CNAP1 Chromosome condensation-related SMC-associated protein 1 −1.65 No No 
        Hs.350966 PTTG1* Securin (pituitary tumor-transforming 1) −1.63 Yes34-36  Yes29,31,36  
        Hs.521097 PTTG3 Pituitary tumor-transforming 3 −1.62 No No 
        Hs.511755 PTTG2 Pituitary tumor-transforming 2 −1.52 No No 
    Mitotic spindle checkpoint      
        Hs.421956 SPC25* Kinetochore protein Spc25 homologue (S cerevisiae) −2.04 Yes36  Yes29,31,36  
        Hs.234545 CDCA1 Kinetochore protein Nuf2 (cell division cycle-associated 1) −1.91 No No 
        Hs.533185 MAD2L1* Mitotic spindle assembly checkpoint protein MAD2 (MAD2-like 1) −1.91 Yes35,36  Yes31,36  
        Hs.36708 BUB1B* Mitotic checkpoint serine/threonine-protein kinase BUBR1 −1.85 Yes33,34,36  Yes29,31,36  
        Hs.414407 KNTC2* Kinetochore protein Hec1 (kinetochore-associated 2) −1.79 Yes35,36  Yes31,36  
        Hs.469649 BUB1* Mitotic checkpoint serine/threonine-protein kinase BUB1 −1.76 No Yes29-31  
        Hs.524947 CDC20* CDC20 cell division cycle 20 homologue (S cerevisiae) −1.6 Yes34  Yes29-31  
    Mitotic spindle assembly      
        Hs.121028 ASPM Abnormal spindle (asp)-like, microcephaly associated (Drosophila) −2.05 No No 
        Hs.514033 SPAG5 Mitotic spindle coiled-coil-related protein SPAG5 (MAP126) −1.91 No No 
        Hs.77695 DLG7* HURP/KIAA0008, discs large homologue 7 (Drosophila) −1.9 No Yes29,31  
        Hs.104019 TACC3 Transforming, acidic coiled-coil-containing protein 3 −1.8 No No 
        Hs.119324 KIF22* Kinesin family member 22 (KNSL4, kinesin-like 4) −1.76 Yes35,36  Yes29,31,36  
        Hs.90073 CSEIL* CSE1 chromosome segregation 1–like (yeast) −1.63 No Yes31  
        Hs.514527 BIRC5* Survivin (baculoviral IAP repeat-containing 5) −1.62 Yes36  Yes31,36  
        Hs.72550 HMMR* Hyaluronan-mediated motility receptor (RHAMM) −1.6 Yes35  Yes29,31  
        Hs.73625 KIF20A* Kinesin family member 20A (RAB6KIFL) −1.59 Yes36  Yes29,30,36  
        Hs.532793 KPNB1* Karyopherin (importin) beta 1 −1.58 Yes36  Yes36  
        Hs.69360 KIF2C* Kinesin family member 2C (KNSL6, kinesin-like 6) −1.53 Yes36  Yes31,36  
    Cytokinesis      
        Hs.126774 RAMP RA-regulated nuclear matrix-associated protein −1.69 No No 
        Hs.459362 PRCI* Protein regulator of cytokinesis 1 −1.66 Yes35,36  Yes29,36  
        Hs.62180 ANLN* Anillin, actin binding protein (scraps homologue, Drosophila) −1.61 Yes35,36  Yes29,36  
    Cell cycle/cell proliferation miscellaneous      
        Hs.470654 CDCA7 Cell division cycle-associated 7 (c-myc target gene JPO1) −2.55 No No 
        Hs.104741 PBK PDZ binding kinase (mitotic kinase TOPK) −1.89 No No 
        Hs.434886 CDCA5 Cell division cycle-associated 5 −1.88 No No 
        Hs.436187 TRIP13* Thyroid hormone receptor interactor 13 −1.78 No Yes30,31  
        Hs.239 FOXM1* Forkhead box M1 −1.65 No Yes31  
        Hs.89497 LMNB1* Lamin B1 −1.65 Yes33  Yes29-31  
        Hs.484813 DEK* DEK oncogene (DNA binding) −1.61 Yes32  Yes32  
        Hs.208912 C22orf18 Proliferation-associated nuclear element (PANE1) −1.56 No Yes29,36  
    Miscellaneous      
        Hs.421907 GLTSCR2 Glioma tumor suppressor candidate region gene 2 −4.41 No No 
        Hs.528306 U1SNRNPBP U11/U12 snRNP 35K −3.77 No No 
        Hs.497636 LAMB3 Laminin, beta 3 −2.91 No No 
        Hs.160411 TSHR Thyroid-stimulating hormone receptor −2.55 No No 
        Hs.7432 THAP1 THAP domain-containing, apoptosis-associated protein 1 −2.13 No No 
        Hs.248941 TAF9 TAF9 RNA polymerase II −2.08 No No 
        Hs.472054 C20orf42 Chromosome 20 open reading frame 42 (kindlerin KIND1) −1.86 No No 
        Hs.71827 RRS1 RRS1 ribosome biogenesis regulator homologue (S cerevisiae) −1.84 No No 
        Hs.296310 GJA4 Gap junction protein, alpha 4, 37kDa (connexin 37) −1.68 No No 
        Hs.472119 MKKS McKusick-Kaufman syndrome −1.62 No No 
        Hs.166539 ITGB3BP Integrin beta 3-binding protein (beta3-endonexin, NRIF3) −1.55 No No 
    Predicted proteins with unknown functions      
        Hs.327252 LZIC Leucine zipper and CTNNB1P1 domain containing −2.18 No No 
        Hs.388087 ZCSL2 Zinc finger, CSL domain-containing 2 −2.07 No No 
        Hs.525764 ARHGAP11A Rho GTPase activating protein 11A −2.03 No No 
        Hs.529778 FKSG14* Leucine zipper protein FKSG14 −2.01 Yes32  Yes32  
        Hs.508292 FLJ10514 Hypothetical protein FLJ10514 −1.89 No No 
        Hs.404323 FLJ10156 Hypothetical protein FLJ10156 −1.81 No No 
        Hs.121536 FAM54A Family with sequence similarity 54, member A −1.79 No No 
        Hs.162717 MGC15668 Hypothetical protein MGC15668 −1.7 No No 
        Hs.330663 FLJ20641 Hypothetical protein FLJ20641 −1.66 No No 
        Hs.127767 LOC130502 Similar to CG14894-PA −1.63 No No 
        Hs.426696 FLJ20516 Timeless-interacting protein −1.62 No No 
        Hs.209715 FLJ22624 FLJ22624 protein −1.6 No No 
        Hs.283532 BM039 Uncharacterized bone marrow protein BM039 −1.58 No No 
        Hs.550564 RPL7L1 Ribosomal protein L7-like 1 −1.53 No No 
        Hs.30696 TCFL5 Transcription factor-like 5 (basic helix-loop-helix) −1.5 No No 
Genes up-regulated after ectopic expression of THAP1 in primary EC 
    Miscellaneous      
        Hs.531081 LGALS3 Lectin, galactoside-binding, soluble, 3 (galectin 3) 1.52 No No 
        Hs.198308 WRB Tryptophan-rich basic protein 1.58 No No 
        Hs.387794 CHGN Chondroitin beta 1,4 N-acetylgalactosaminyltransferase 1.6 No No 
        Hs.45140 TMEM35 Transmembrane protein 35 1.65 No No 
        Hs.505141 FLJ14337 Similar to peptidylprolyl isomerase A, isoform 1 1.67 No No 
        Hs.549129 RP11-529110.4 Deleted in a mouse model of primary ciliary dyskinesia 1.69 No No 
        Hs.508835 TEP1 Telomerase-associated protein 1 1.7 No No 
        Hs.518731 UCHL1 Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) 1.71 No No 
        Hs.106511 PCDH17 Protocadherin 17 1.71 No No 
        Hs.492261 TP53INP1 Tumor protein p53 inducible nuclear protein 1 1.89 No No 
        Hs.126598 TMEM45A Transmembrane protein 45A 1.96 No No 
        Hs.391561 FABP4 Fatty acid binding protein 4, adipocyte 1.98 No No 
        Hs.386470 NMB Neuromedin B 2.01 No No 
        Hs.517581 HMOX1 Heme oxygenase (decycling) 1 3.06 No No 
        Hs.289292 FOXL2 Forkhead box L2 4.21 No No 
        Hs.546296 SECTM1 Secreted and transmembrane 1 5.51 No No 

The Unigene ID, gene symbol, gene name, and description are indicated for each gene as well as the fold changes obtained after 2 retroviral transductions of HUVECs with pMLV-MCS or pMLV-THAP1 expression vectors (#2xTHAPI experiment). The table includes only those genes reproducibly affected by THAP1 expression (P < .01) in the 2 fully independent microarray experiments (#lxTHAP1 and #2xTHAP1).

*

Genes previously identified as targets of the pRB/E2F pathway.

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