Table 5 Production of TNF-α and IFN-γ by... | American Society of Hematology

Table 5

Production of TNF-α and IFN-γ by CD4⁺CD25⁻ T lymphocytes (Teffs) alone and in the presence of CD4⁺CD25⁺ regulatory T cells (Tregs) at a 1:1 ratio

	TNF-α	IFN-γ
Teffs	<5.0	<2.0
Teffs + PHA	542.5 ± 130.5*	671.1 ± 18.4*
Teffs + Tregs	< 5.0	< 2.0
Teffs + Tregs + PHA	272.9 ± 93.3†	301.6 ± 65.5†
Teffs + Tregs + PHA + reserpine	304.3 ± 112.5‡	255.3 ± 101.8†
Tregs	< 5.0	< 2.0
Tregs + PHA	26.9 ± 46.6	40.4 ± 68.62

	TNF-α	IFN-γ
Teffs	<5.0	<2.0
Teffs + PHA	542.5 ± 130.5*	671.1 ± 18.4*
Teffs + Tregs	< 5.0	< 2.0
Teffs + Tregs + PHA	272.9 ± 93.3†	301.6 ± 65.5†
Teffs + Tregs + PHA + reserpine	304.3 ± 112.5‡	255.3 ± 101.8†
Tregs	< 5.0	< 2.0
Tregs + PHA	26.9 ± 46.6	40.4 ± 68.62

Cells were cultured for 48 hours under standard conditions in the absence or in the presence of 1 μ reserpine. Data are mean α SD of n = 3 separate observations and are expressed as pg/mL.

P < .001 versus Teffs alone.

†

P < .01 versus Teffs + PHA.

‡

P < .05 versus Teffs + PHA.

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