Relative concentrations of Slc35d3 mRNA in tissues of C3H and ash-Roswell mutants
| Tissues . | ΔCt values . | Relative Slc35d3 mRNA level (ash-Roswell/C3H) . | |
|---|---|---|---|
| C3H . | Ash/R . | ||
| Brain | 15.8 ± 0.17 | 12.7 ± 0.40* | 8 |
| Spleen | 15.9 ± 0.76 | 11.1 ± 0.34† | 32 |
| Bone marrow | 15.9 ± 0.37 | 11.8 ± 0.37* | 16 |
| Adult liver | 24.1 ± 0.24 | 13.3 ± 0.31‡ | 2048 |
| Kidney | 25.6 ± 0.51 | 14.1 ± 0.34‡ | 2432 |
| Lungs | 18.7 ± 0.56 | 10.6 ± 0.17‡ | 256 |
| Platelets | 12.1 | 3.0 | 512 |
| Tissues . | ΔCt values . | Relative Slc35d3 mRNA level (ash-Roswell/C3H) . | |
|---|---|---|---|
| C3H . | Ash/R . | ||
| Brain | 15.8 ± 0.17 | 12.7 ± 0.40* | 8 |
| Spleen | 15.9 ± 0.76 | 11.1 ± 0.34† | 32 |
| Bone marrow | 15.9 ± 0.37 | 11.8 ± 0.37* | 16 |
| Adult liver | 24.1 ± 0.24 | 13.3 ± 0.31‡ | 2048 |
| Kidney | 25.6 ± 0.51 | 14.1 ± 0.34‡ | 2432 |
| Lungs | 18.7 ± 0.56 | 10.6 ± 0.17‡ | 256 |
| Platelets | 12.1 | 3.0 | 512 |
Quantitative, real-time PCR analyses of mRNA concentrations were determined using TaqMan probes. Expression levels were standardized to levels of GAPDH mRNA by calculating cycle thresholds (Ct). ΔCt = Slc35D3 Ct − GAPDH Ct. Values are mean ± SEM of 3 separate mouse samples. RNA was combined from the platelets isolated from 3 mice.
P < .01 compared with same tissue of C3H mice.
P < .02 compared with same tissue of C3H mice.
P < .001 compared with same tissue of C3H mice.