Reverse transcription–polymerase chain reaction mouse primer sequences information
| . | Primer pair sequence . | . | . | |
|---|---|---|---|---|
| Primer . | Forward . | Reverse . | Annealing temperature, °C . | Product size, bp . |
| β-actin | GTGGGGCGCCCCAGGCACCA | CTCCTTAATGTCACGCACGATTTC | 50 | 520 |
| PPAR-γ2 | GGAGATTCTCCTGTTGACCCAG | GGCACTCAATGGCCATGAG | 65 | 403 |
| C/EBP-α | GATAAAGCCAAACAACGCAACG | CTAGAGATCCAGCGACCCGAA | 50 | 253 |
| LPL | TTAACTACCCCCTAGACAACGTCCA | AAGAGATGAATGGAGCGCTCG | 50 | 388 |
| Adipsin | AGACCCCTACCCTTGCAATACG | TGTTACCATTTGTGATGTTTTCGATC | 50 | 376 |
| OPN | TGGAGATCGAATTCTGCTTG | TCAAGTGCTTGAGGGCATAC | 50 | 720 |
| Cbfa-1 | AGTAGCCAGGTTCAACGATCTGA | GGACCGTCCACTGTCACTTTAATA | 50 | 137 |
| Coll II | GGCAACAGCAGGTTCACATA | CCACACCAAATTCCTGTTCA | 55 | 166 |
| Agg | CTAAGTTCCAGGGTCACTGTTACCG | TCCTCTCCGGTGGCAAAGAAGTTG | 59 | 270 |
| Coll X | AAGGAGTGCCTGGACACAAT | GTCGTAATGCTGCTGCCTAT | 55 | 461 |
| . | Primer pair sequence . | . | . | |
|---|---|---|---|---|
| Primer . | Forward . | Reverse . | Annealing temperature, °C . | Product size, bp . |
| β-actin | GTGGGGCGCCCCAGGCACCA | CTCCTTAATGTCACGCACGATTTC | 50 | 520 |
| PPAR-γ2 | GGAGATTCTCCTGTTGACCCAG | GGCACTCAATGGCCATGAG | 65 | 403 |
| C/EBP-α | GATAAAGCCAAACAACGCAACG | CTAGAGATCCAGCGACCCGAA | 50 | 253 |
| LPL | TTAACTACCCCCTAGACAACGTCCA | AAGAGATGAATGGAGCGCTCG | 50 | 388 |
| Adipsin | AGACCCCTACCCTTGCAATACG | TGTTACCATTTGTGATGTTTTCGATC | 50 | 376 |
| OPN | TGGAGATCGAATTCTGCTTG | TCAAGTGCTTGAGGGCATAC | 50 | 720 |
| Cbfa-1 | AGTAGCCAGGTTCAACGATCTGA | GGACCGTCCACTGTCACTTTAATA | 50 | 137 |
| Coll II | GGCAACAGCAGGTTCACATA | CCACACCAAATTCCTGTTCA | 55 | 166 |
| Agg | CTAAGTTCCAGGGTCACTGTTACCG | TCCTCTCCGGTGGCAAAGAAGTTG | 59 | 270 |
| Coll X | AAGGAGTGCCTGGACACAAT | GTCGTAATGCTGCTGCCTAT | 55 | 461 |
PPAR-γ2 indicates peroxisome proliferator-activated receptor γ 2; C/EBP-α, CCAAT/enhancer-binding protein-α; LPL, lipoprotein lipase; OPN, osteopontin; Cbfa-1, core-binding factor-1, Coll II, collagen II; Agg, aggrecan; Coll X, collagen X.