Notch1 mutations and tumorigenicity in preleukemic thymocytes
. | . | . | . | Nude mice . | . | . | ||
|---|---|---|---|---|---|---|---|---|
| Mouse . | Age, wk . | Notch1 mutation . | Injection into nude mice . | Tumor . | Tcrb rgmt . | Notch1 mutation . | ||
| 7162/3 | 5 | None | − | NA | NA | NA | ||
| 7543/3 | 5 | None | + | − | − | NA | ||
| 7544/2 | 5 | None | + | − | − | NA | ||
| 7286/1 | 8 | 66601CCCC>CCCCC | + | + | + | 66601CCCC>CCCCC | ||
| 7193/3 | 10 | 66569GACCCC>CCATGACTCCT | − | NA | NA | NA | ||
| 7151/4 | 12 | 66419C>GG | + | + | + | 66419C>GG | ||
| 7150/3 | 12 | 66456C>CC | + | + | + | 66456C>CC | ||
. | . | . | . | Nude mice . | . | . | ||
|---|---|---|---|---|---|---|---|---|
| Mouse . | Age, wk . | Notch1 mutation . | Injection into nude mice . | Tumor . | Tcrb rgmt . | Notch1 mutation . | ||
| 7162/3 | 5 | None | − | NA | NA | NA | ||
| 7543/3 | 5 | None | + | − | − | NA | ||
| 7544/2 | 5 | None | + | − | − | NA | ||
| 7286/1 | 8 | 66601CCCC>CCCCC | + | + | + | 66601CCCC>CCCCC | ||
| 7193/3 | 10 | 66569GACCCC>CCATGACTCCT | − | NA | NA | NA | ||
| 7151/4 | 12 | 66419C>GG | + | + | + | 66419C>GG | ||
| 7150/3 | 12 | 66456C>CC | + | + | + | 66456C>CC | ||
Thymocytes from young, clinically healthy SCL/LMO1 mice were transplanted into nude mice. Thymocytes from mice 8 weeks and older had Notch1 mutations and formed tumors in nude mice. Notch1 mutations are insertion/deletions; the numbers refer to the Notch1 reference sequence (GenBank no. AL732541.11).
Tcrb rgmt indicates clonal Tcrb rearrangement by Southern blot analysis; and NA, not applicable.