Average response of the TLR-deficient EFs and WT-EFs to IgG purified from 6 patients with APS, 4 patients with autoimmune diseases, and from a pool of healthy controls
| . | TLR1−/−EF . | TLR2−/−EF . | TLR4−/−EF . | TLR6−/−EF . | WT-EF . |
|---|---|---|---|---|---|
| APLA | |||||
| MCP-1 | 2.64 ± 0.87 | 1.16 ± 0.1* | 27.85 ± 5.27 | 4.50 ± 2.33 | 16.02 ± 6.5 |
| ICAM-1 | 2.95 ± 1.25 | 1.90 ± 0.24* | 23.70 ± 7.25 | 3.55 ± 0.94 | 8.31 ± 2.02 |
| IL-6 | 2.80 ± 0.74 | 1.70 ± 0.21* | 23.90 ± 7.69 | 13.86 ± 7.9 | 18.35 ± 9 |
| AI IgG | |||||
| MCP-1 | 0.81 ± 0.1 | 0.90 ± 0.16 | 1.58 ± 0.24 | 0.48 ± 0.6 | 1.0 ± 0.1 |
| ICAM-1 | 1.04 ± 0.17 | 1.80 ± 0.36 | 2.66 ± 0.63 | 0.47 ± 0.18 | 1.81 ± 0.38 |
| IL-6 | 0.84 ± 0.1 | 0.73 ± 0.13 | 1.57 ± 0.24 | 0.43 ± 0.19 | 1.10 ± 0.2 |
| Control IgG | |||||
| MCP-1 | 0.85 ± 0.11 | 0.65 ± 0.14 | 1.12 ± 0.14 | 0.78 ± 0.22 | 1.15 ± 0.04 |
| ICAM-1 | 0.90 ± 0.14 | 0.66 ± 0.27 | 1.03 ± 0.43 | 1.40 ± 0.4 | 1.15 ± 0.03 |
| IL-6 | 1.23 ± 0.32 | 1.05 ± 0.45 | 1.28 ± 0.36 | 0.70 ± 0.28 | 1.55 ± 0.25 |
| . | TLR1−/−EF . | TLR2−/−EF . | TLR4−/−EF . | TLR6−/−EF . | WT-EF . |
|---|---|---|---|---|---|
| APLA | |||||
| MCP-1 | 2.64 ± 0.87 | 1.16 ± 0.1* | 27.85 ± 5.27 | 4.50 ± 2.33 | 16.02 ± 6.5 |
| ICAM-1 | 2.95 ± 1.25 | 1.90 ± 0.24* | 23.70 ± 7.25 | 3.55 ± 0.94 | 8.31 ± 2.02 |
| IL-6 | 2.80 ± 0.74 | 1.70 ± 0.21* | 23.90 ± 7.69 | 13.86 ± 7.9 | 18.35 ± 9 |
| AI IgG | |||||
| MCP-1 | 0.81 ± 0.1 | 0.90 ± 0.16 | 1.58 ± 0.24 | 0.48 ± 0.6 | 1.0 ± 0.1 |
| ICAM-1 | 1.04 ± 0.17 | 1.80 ± 0.36 | 2.66 ± 0.63 | 0.47 ± 0.18 | 1.81 ± 0.38 |
| IL-6 | 0.84 ± 0.1 | 0.73 ± 0.13 | 1.57 ± 0.24 | 0.43 ± 0.19 | 1.10 ± 0.2 |
| Control IgG | |||||
| MCP-1 | 0.85 ± 0.11 | 0.65 ± 0.14 | 1.12 ± 0.14 | 0.78 ± 0.22 | 1.15 ± 0.04 |
| ICAM-1 | 0.90 ± 0.14 | 0.66 ± 0.27 | 1.03 ± 0.43 | 1.40 ± 0.4 | 1.15 ± 0.03 |
| IL-6 | 1.23 ± 0.32 | 1.05 ± 0.45 | 1.28 ± 0.36 | 0.70 ± 0.28 | 1.55 ± 0.25 |
Data are expressed as the ratio (mean ± SE) of mRNA levels of the inflammatory marker proteins MCP-1, ICAM-1, and IL-6 in nonstimulated cells compared with IgG-stimulated cells.
AI indicates autoimmune.
Statistically different from WT-EFs and TLR4−/−EFs (P < .001).