Oligonucleotides used for RT-PCR, real-time PCR, and ChIP analyses
| . | Sense primer, 5′→3′ . | Antisense primer, 5′→3′ . |
|---|---|---|
| RT-PCR/real-time PCR | ||
| HoxA9 | ||
| RT-PCR | gcccggtgcgctctccttcgc | catcctgcggttctggaaccag |
| Real-time PCR | aaaaacaacccagcgaa | caaatggcatcactcgtc |
| HoxB3 | ctgctctcttcggaggctattc | ctgttgctagtggcactggtagg |
| HoxB4 | gtcccactccgcgtgcaaagag | cagagcgcgtgggcgatctcc |
| HoxB5 | ggcttcgagctgctccctgtcc | ggctgatgtgaagcttcctcatcc |
| HoxB9 | gcacgaagtggccagactcctc | gctggaagtgaggggctaggac |
| HoxD3 | agaaggctgcttactatgaaaaccc | acaagtagcggttgaagtggaattc |
| MLL | cagctatcctctcagatccatc | ccttgtctttccggactttctg |
| EphB4 | cctgctatccctgcacctcttc | ctgatccaatggtgttagagtgg |
| Integrin αV | 5′-gggattttgtcaaggaggattc-3′ | 5′-ccaaagtccttgctgctcttgg-3′ |
| Integrin α5 | gaccagagttacgggactcaactg | cttggtccattgcacagctgtgg |
| Integrin β1 | tgagtgcaaccccaactacactg | gtctggaccagtgggacactctg |
| Integrin β3 | gtgcggcaggtggaggattac | cacgtgtttgtagccaaacatgg |
| Meis1 | ggtacgacgatctaccccattac | gtcattgacagaggagcccatgc |
| GAPDH | ||
| RT-PCR | tcaccatcttccaggagcgagatc | gagaccacctggtgctcagtgtag |
| Real-time PCR | ttggtatcgtggaaggactca | tgtcatcatatttggcaggttt |
| ChIP | ||
| HoxA9 P1 | aatgcgatttggctgcttttttatg | tcaaatctagccttgtctctgtac |
| HoxA9 P2 | gggagacgggagagtacagagac | cgtccagcagaacaataacgcg |
| HoxA9 P3 | caactactacgtggactcgttc | gtgatggtggtggtacaccgc |
| HoxD3 P4 | gtagtggattgtcattgagtggg | cctgcctcccaagttgacaaag |
| HoxD3 P5 | ggttaggctgtttggtgcaggtg | cataggtcagctccctggtctc |
| HoxD3 P6 | cacctccttagtccccctggtc | ctgtggtgaggactgtggtttttg |
| . | Sense primer, 5′→3′ . | Antisense primer, 5′→3′ . |
|---|---|---|
| RT-PCR/real-time PCR | ||
| HoxA9 | ||
| RT-PCR | gcccggtgcgctctccttcgc | catcctgcggttctggaaccag |
| Real-time PCR | aaaaacaacccagcgaa | caaatggcatcactcgtc |
| HoxB3 | ctgctctcttcggaggctattc | ctgttgctagtggcactggtagg |
| HoxB4 | gtcccactccgcgtgcaaagag | cagagcgcgtgggcgatctcc |
| HoxB5 | ggcttcgagctgctccctgtcc | ggctgatgtgaagcttcctcatcc |
| HoxB9 | gcacgaagtggccagactcctc | gctggaagtgaggggctaggac |
| HoxD3 | agaaggctgcttactatgaaaaccc | acaagtagcggttgaagtggaattc |
| MLL | cagctatcctctcagatccatc | ccttgtctttccggactttctg |
| EphB4 | cctgctatccctgcacctcttc | ctgatccaatggtgttagagtgg |
| Integrin αV | 5′-gggattttgtcaaggaggattc-3′ | 5′-ccaaagtccttgctgctcttgg-3′ |
| Integrin α5 | gaccagagttacgggactcaactg | cttggtccattgcacagctgtgg |
| Integrin β1 | tgagtgcaaccccaactacactg | gtctggaccagtgggacactctg |
| Integrin β3 | gtgcggcaggtggaggattac | cacgtgtttgtagccaaacatgg |
| Meis1 | ggtacgacgatctaccccattac | gtcattgacagaggagcccatgc |
| GAPDH | ||
| RT-PCR | tcaccatcttccaggagcgagatc | gagaccacctggtgctcagtgtag |
| Real-time PCR | ttggtatcgtggaaggactca | tgtcatcatatttggcaggttt |
| ChIP | ||
| HoxA9 P1 | aatgcgatttggctgcttttttatg | tcaaatctagccttgtctctgtac |
| HoxA9 P2 | gggagacgggagagtacagagac | cgtccagcagaacaataacgcg |
| HoxA9 P3 | caactactacgtggactcgttc | gtgatggtggtggtacaccgc |
| HoxD3 P4 | gtagtggattgtcattgagtggg | cctgcctcccaagttgacaaag |
| HoxD3 P5 | ggttaggctgtttggtgcaggtg | cataggtcagctccctggtctc |
| HoxD3 P6 | cacctccttagtccccctggtc | ctgtggtgaggactgtggtttttg |
RT-PCR indicates reverse transcription-polymerase chain reaction.