Similar levels of mixed chimerism in GalT−/− and GalT−/−CR2−/− mice after nonmyeloablative conditioning and H-2-mismatched BMT
| Group (donor and recipient)* . | No. . | Percentage of donor cells† . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CD4 . | B cells . | Monocytes . | ||||||||
| 4 wk after preparation . | 8 wk after preparation . | 16 wk after preparation . | 4 wk after preparation . | 8 wk after preparation . | 16 wk after preparation . | 4 wk after preparation . | 8 wk after preparation . | 16 wk after preparation . | ||
| A (BALB/c and GalTKO) | 9 | 33.30 ± 27.70 | 24.70 ± 20.30 | 25.80 ± 17.60 | 41.30 ± 28.40 | 44.20 ± 18.60 | 42.90 ± 14.30 | 58.3 ± 17.7 | 48.6 ± 18.6 | 44.5 ± 12.8 |
| B (BALB/c and GalTCR2DKO) | 7 | 12.80 ± 5.2‡ | 42.40 ± 19.3‡ | 40.90 ± 18.4‡ | 56.40 ± 22.5‡ | 59.00 ± 20.7‡ | 62.40 ± 17.2§ | 53.8 ± 14.5‡ | 39.6 ± 15.1‡ | 40.5 ± 12.4‡ |
| Group (donor and recipient)* . | No. . | Percentage of donor cells† . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| CD4 . | B cells . | Monocytes . | ||||||||
| 4 wk after preparation . | 8 wk after preparation . | 16 wk after preparation . | 4 wk after preparation . | 8 wk after preparation . | 16 wk after preparation . | 4 wk after preparation . | 8 wk after preparation . | 16 wk after preparation . | ||
| A (BALB/c and GalTKO) | 9 | 33.30 ± 27.70 | 24.70 ± 20.30 | 25.80 ± 17.60 | 41.30 ± 28.40 | 44.20 ± 18.60 | 42.90 ± 14.30 | 58.3 ± 17.7 | 48.6 ± 18.6 | 44.5 ± 12.8 |
| B (BALB/c and GalTCR2DKO) | 7 | 12.80 ± 5.2‡ | 42.40 ± 19.3‡ | 40.90 ± 18.4‡ | 56.40 ± 22.5‡ | 59.00 ± 20.7‡ | 62.40 ± 17.2§ | 53.8 ± 14.5‡ | 39.6 ± 15.1‡ | 40.5 ± 12.4‡ |
Recipient GalT−/− or GalT−/−Cr2−/− mice were treated with anti-CD4 and anti-CD8 mAbs on day –5, TI and TBI on day 0, and BALB/c BMT on day 0 with 2 × 107 cells.
Chimerism was detected by flow cytometric analysis after staining with FITC-conjugated anti-CD4, anti-CD19, and anti-Mac 1 mAbs, biotinylated anti-H-2Dd mAb, and PE-streptavidin. To analyze CD4 and B-cell chimerism, a lymphocyte forward scatter/side scatter (FSC/SSC) gate was set. To analyze monocyte chimerism, a monocyte FSC/SSC gate was set. The mean ± SD percentage of donor cells in each lineage is shown.
NS compared with GalT−/− chimeras at the same time point.
P < .05 compared with GalT−/− chimeras at the same time point.