TLR-specific primers
| TLR . | Primer pairs . | Tm0 . | Product size, bp . | |
|---|---|---|---|---|
| Sense . | Antisense . | |||
| TLR-1 | GCGAGCAGAGGCAATTGTGGA | GACAGAGCCTGTAAGCATATTCG | 51 | 428 |
| TLR-2 | CGGTCAGAAAACAACTTACCGAA | TACCCAGCTCGCTCACTACGT | 60 | 975 |
| TLR-3 | TTGTCTTCTGCACGAACCTG | CGCAACGCAAGGATTTTATT | 53 | 207 |
| TLR-4 | CAAGAACATAGATCTGAGCTTCAACCC | GCTGTCCAATAGGGAAGCTTTCTAGAG | 57 | 280 |
| TLR-5 | ACTGAATTCCTTAAGCGACGTA | AGAAGATAAAGCCGTGCGAAA | 51 | 428 |
| TLR-6 | GTACCGTCAGTGCTGGAAATA | CCAGGAAAGTCAGCTTCGTC | 47 | 543 |
| TLR-7 | TTCCGATACGATGAATATGCACG | TGAGTTTGTCCAGAAGCCGTAAT | 51 | 404 |
| TLR-8 | CGTTTTACCTTCCTTTGTCT | CATTTGGGTGCTGTTGTTTG | 51 | 342 |
| TLR-9 | GGAGAATCCTCCATCTCCCAA | CCAGGAAGGTTCTGGGCTCA | 54 | 386 |
| GAPDH | AACTTTGGCATTGTGGAAGG | ACACATTGGGGGTAGGAACA | 55 | 225 |
| TLR . | Primer pairs . | Tm0 . | Product size, bp . | |
|---|---|---|---|---|
| Sense . | Antisense . | |||
| TLR-1 | GCGAGCAGAGGCAATTGTGGA | GACAGAGCCTGTAAGCATATTCG | 51 | 428 |
| TLR-2 | CGGTCAGAAAACAACTTACCGAA | TACCCAGCTCGCTCACTACGT | 60 | 975 |
| TLR-3 | TTGTCTTCTGCACGAACCTG | CGCAACGCAAGGATTTTATT | 53 | 207 |
| TLR-4 | CAAGAACATAGATCTGAGCTTCAACCC | GCTGTCCAATAGGGAAGCTTTCTAGAG | 57 | 280 |
| TLR-5 | ACTGAATTCCTTAAGCGACGTA | AGAAGATAAAGCCGTGCGAAA | 51 | 428 |
| TLR-6 | GTACCGTCAGTGCTGGAAATA | CCAGGAAAGTCAGCTTCGTC | 47 | 543 |
| TLR-7 | TTCCGATACGATGAATATGCACG | TGAGTTTGTCCAGAAGCCGTAAT | 51 | 404 |
| TLR-8 | CGTTTTACCTTCCTTTGTCT | CATTTGGGTGCTGTTGTTTG | 51 | 342 |
| TLR-9 | GGAGAATCCTCCATCTCCCAA | CCAGGAAGGTTCTGGGCTCA | 54 | 386 |
| GAPDH | AACTTTGGCATTGTGGAAGG | ACACATTGGGGGTAGGAACA | 55 | 225 |
Tm0 indicates melting temperature in degrees Celsius.