Expression of IL-1–signaling molecules by embryonic stromal cell lines
| Cell line . | Origin . | Expression . | |||||
|---|---|---|---|---|---|---|---|
| IL-1RI(Il1r1) . | IL-1RacP(Il1rap) . | IL-1RII(Il1r2) . | IL-1a(Il1a) . | IL-1b(Il1b) . | Actin(Actb) . | ||
| AM20-1A4 | Ao | ++ | ++ | ++ | − | − | ++ |
| AM30-2A4 | Ao | ++ | ++ | ± | − | − | ++ |
| AM30-3F4 | Ao | ++ | ++ | − | − | − | ++ |
| AM30-3A3 | Ao | ++ | ++ | + | − | − | + |
| UG26-1B6 | UGR | ++ | ++ | − | − | − | + |
| EL08-1D2 | FL | ++ | ++ | − | − | − | + |
| EL23-1C2 | FL | ++ | ++ | − | − | − | + |
| FBMD-1 | BM | ++ | ++ | − | + | − | ++ |
| Cell line . | Origin . | Expression . | |||||
|---|---|---|---|---|---|---|---|
| IL-1RI(Il1r1) . | IL-1RacP(Il1rap) . | IL-1RII(Il1r2) . | IL-1a(Il1a) . | IL-1b(Il1b) . | Actin(Actb) . | ||
| AM20-1A4 | Ao | ++ | ++ | ++ | − | − | ++ |
| AM30-2A4 | Ao | ++ | ++ | ± | − | − | ++ |
| AM30-3F4 | Ao | ++ | ++ | − | − | − | ++ |
| AM30-3A3 | Ao | ++ | ++ | + | − | − | + |
| UG26-1B6 | UGR | ++ | ++ | − | − | − | + |
| EL08-1D2 | FL | ++ | ++ | − | − | − | + |
| EL23-1C2 | FL | ++ | ++ | − | − | − | + |
| FBMD-1 | BM | ++ | ++ | − | + | − | ++ |
IL-1R (IL-1RI and IL-1RAcP) expression is detected RT-PCR in several stromal cell lines derived from different embryonic and adult mouse tissues. No IL-1β expression could be detected in these cell lines and IL-1α expression was only detected in the adult BM-derived FBMD-1 stromal cell line. Expression of the IL-1 decoy receptor (IL-1RII) was detected in some of the stromal cell lines tested. Expression levels of these transcripts are indicated by their relative expression levels, with ++ indicating very high expression; +, high expression; ±, low expression; and −, no expression detected.
BM indicates bone marrow; Ao, dorsal aorta from AGM region; UGR, urogenital ridge from AGM region; and FL, fetal liver.