In contrast with B6 WT, primed B-cell-deficient recipients engraft if given high donor BM cell doses
| Recipient and no. BM cells . | Survival . | No. engrafted . | Mean % donor . |
|---|---|---|---|
| B6 WT, 40 × 106 | |||
| Unprimed | 10/10 | 10/10 | 98 ± 2 |
| Primed | 0/10* | NA | NA |
| B6 WT, 200 × 106 | |||
| Unprimed | 4/4 | 4/4 | 100 ± 0 |
| Primed | 3/4 | 0/3* | 0 ± 0* |
| B6 muMT, 200 × 106 | |||
| Unprimed | 4/4 | 4/4 | 99 ± 1 |
| Primed | 4/4 | 4/4 | 100 ± 2 |
| B6 muMT, 100 × 106 | |||
| Unprimed | 4/4 | 4/4 | 98 ± 3 |
| Primed | 4/4 | 4/4 | 100 ± 0 |
| B6 muMT, 20 × 106 | |||
| Unprimed | 8/8 | 8/8 | 83 ± 11 |
| Primed | 11/12 | 2/11* | 16 ± 11* |
| Recipient and no. BM cells . | Survival . | No. engrafted . | Mean % donor . |
|---|---|---|---|
| B6 WT, 40 × 106 | |||
| Unprimed | 10/10 | 10/10 | 98 ± 2 |
| Primed | 0/10* | NA | NA |
| B6 WT, 200 × 106 | |||
| Unprimed | 4/4 | 4/4 | 100 ± 0 |
| Primed | 3/4 | 0/3* | 0 ± 0* |
| B6 muMT, 200 × 106 | |||
| Unprimed | 4/4 | 4/4 | 99 ± 1 |
| Primed | 4/4 | 4/4 | 100 ± 2 |
| B6 muMT, 100 × 106 | |||
| Unprimed | 4/4 | 4/4 | 98 ± 3 |
| Primed | 4/4 | 4/4 | 100 ± 0 |
| B6 muMT, 20 × 106 | |||
| Unprimed | 8/8 | 8/8 | 83 ± 11 |
| Primed | 11/12 | 2/11* | 16 ± 11* |
B6 WT or B6 muMT mice were primed on day −28 with 20 × 106 BALB/c splenocytes intraperitoneally, irradiated on day −1 (6.0 Gy), and given non-T-cell-depleted BALB/c BM cells at the indicated number on day 0. Survival was monitored. PBLs were phenotyped 6 weeks after transplantation for donor chimerism. All engrafting mice were high-level donor chimeras (> 90%). Shown is percentage of mean group donor chimerism ± 1 SEM.
NA indicates not available.
P < .01 versus nonprimed control at same BM cell dose.