T-cell division following immunization with OVA323-339 peptide in OT-II and OT-II/CD28 Y170F mice
| . | Total CD4+ T cells, % . | No. of divisions, % of total CD4+ T cells . | % divided . | % undivided . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | ||||
| OT-2 C | 36.8 ± 3.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.62 ± 0.62 | 1.42 ± 0.87 |
| OT-2 + Ag | 33.33 ± 1.06 | 0.04 ± 0.01 | 0.12 ± 0.03 | 0.36 ± 0.1 | 0.82 ± 0.3 | 1.27 ± 0.3 | 1.47 ± 0.2 | 1.17 ± 0.12 | 4.90 ± 1.2 | 0.06 ± 0.01 |
| OT-2 Y170F C | 32.1 ± 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.44 ± 0.3 | 0.96 ± 0.7 |
| OT-2 Y170F + Ag | 32.73 ± 0.3 | 0.12 ± 0.03 | 0.18 ± 0.06 | 0.47 ± 0.2 | 0.67 ± 0.3 | 0.96 ± 0.3 | 1.17 ± 0.3 | 1.48 ± 0.2 | 5.08 ± 1.3 | 0.19 ± 0.06 |
| . | Total CD4+ T cells, % . | No. of divisions, % of total CD4+ T cells . | % divided . | % undivided . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 1 . | 2 . | 3 . | 4 . | 5 . | 6 . | 7 . | ||||
| OT-2 C | 36.8 ± 3.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.62 ± 0.62 | 1.42 ± 0.87 |
| OT-2 + Ag | 33.33 ± 1.06 | 0.04 ± 0.01 | 0.12 ± 0.03 | 0.36 ± 0.1 | 0.82 ± 0.3 | 1.27 ± 0.3 | 1.47 ± 0.2 | 1.17 ± 0.12 | 4.90 ± 1.2 | 0.06 ± 0.01 |
| OT-2 Y170F C | 32.1 ± 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.44 ± 0.3 | 0.96 ± 0.7 |
| OT-2 Y170F + Ag | 32.73 ± 0.3 | 0.12 ± 0.03 | 0.18 ± 0.06 | 0.47 ± 0.2 | 0.67 ± 0.3 | 0.96 ± 0.3 | 1.17 ± 0.3 | 1.48 ± 0.2 | 5.08 ± 1.3 | 0.19 ± 0.06 |
Evaluation of CFSE-labeled OT-II and OT-II/Y170F CD4+ T-cell division in draining (mesenteric) lymph nodes 96 hours after immunization with OVA323-339 peptide and LPS intraperitoneally. Cells are gated based on CD4 expression (CD4-APC, FL-2). The total percentage of CD4+ T cells, the percentage of CD4+ CFSE-labeled cells that underwent the indicated number of divisions, and the total number of CD4+ T cells that underwent division or did not divide are indicated in the relevant columns.