Table 2. Immunohistochemical prediction... | American Society of Hematology

Table 2.

Immunohistochemical prediction of exon-12 NPM mutations in 200 adult NPMc⁺ AML patients

Mutation	Nucleotide sequence
WT*	gaccaagaggctattcaagatctct	g	gcag	t	ggaggaagtctctttaagaaaatag	286-DLWQWRKSL		Nucl
A	gaccaagaggctattcaagatctct	gTCTG	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCLAVEEVSLRK	80.5	Cyt
B	gaccaagaggctattcaagatctct	gCATG	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCMAVEEVSLRK	7.5	Cyt
C	gaccaagaggctattcaagatctct	gCGTG	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCVAVEEVSLRK	< 1	Cyt
D	gaccaagaggctattcaagatctct	gCCTG	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCLAVEEVSLRK	5.5	Cyt
E	gaccaagaggctattcaagatctct	g	gcag	tCTCTTGCCC	aagtctctttaagaaaatag	286-DLWQSLAQVSLRK	< 1	Cyt
F	gaccaagaggctattcaagatctct	g	gcag	tCCCTGGAGA	aagtctctttaagaaaatag	286-DLWQSLEKVSLRK	< 1	Cyt
J	gaccaagaggctattcaagatctct	g	gcag	tCTCTTTCTA	aagtctctttaagaaaatag	286-DLWQSLSKVSLRK	< 1	Cyt
L	gaccaagaggctattcaagatctctCCCGg			gcag	t	aagtctctttaagaaaatag	286-DLSRAVEEVSLRK	< 1	Cyt
K	gaccaagaggctattcaagatctct	g	gcag	tCCCTTTCCA	aagtctctttaagaaaatag	286-DLWQSLSKVSLRK	< 1	Cyt
M	gaccaagaggctattcaagatctct	gTAGC	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCTAVEEVSLRK	< 1	Cyt
N	gaccaagaggctattcaagatctct	gCCAC	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCHAVEEVSLRK	< 1	Cyt
O	gaccaagaggctattcaagatctct	g	gcagCGTTTCC		aggaagtctctttaagaaaatag	286-DLWQRFQEVSLRK	< 1	Cyt
P	gaccaagaggctattcaagatctct	gTACCTTCC		t	ggaggaagtctctttaagaaaatag	286-DLCTFLEEVSLRK	< 1	Cyt
Q	gaccaagaggctattcaagatctct	g	gcagAGGA	t	ggaggaagtctctttaagaaaatag	286-DLWQRMEEVSLRK	< 1	Cyt

Mutation	Nucleotide sequence
WT*	gaccaagaggctattcaagatctct	g	gcag	t	ggaggaagtctctttaagaaaatag	286-DLWQWRKSL		Nucl
A	gaccaagaggctattcaagatctct	gTCTG	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCLAVEEVSLRK	80.5	Cyt
B	gaccaagaggctattcaagatctct	gCATG	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCMAVEEVSLRK	7.5	Cyt
C	gaccaagaggctattcaagatctct	gCGTG	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCVAVEEVSLRK	< 1	Cyt
D	gaccaagaggctattcaagatctct	gCCTG	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCLAVEEVSLRK	5.5	Cyt
E	gaccaagaggctattcaagatctct	g	gcag	tCTCTTGCCC	aagtctctttaagaaaatag	286-DLWQSLAQVSLRK	< 1	Cyt
F	gaccaagaggctattcaagatctct	g	gcag	tCCCTGGAGA	aagtctctttaagaaaatag	286-DLWQSLEKVSLRK	< 1	Cyt
J	gaccaagaggctattcaagatctct	g	gcag	tCTCTTTCTA	aagtctctttaagaaaatag	286-DLWQSLSKVSLRK	< 1	Cyt
L	gaccaagaggctattcaagatctctCCCGg			gcag	t	aagtctctttaagaaaatag	286-DLSRAVEEVSLRK	< 1	Cyt
K	gaccaagaggctattcaagatctct	g	gcag	tCCCTTTCCA	aagtctctttaagaaaatag	286-DLWQSLSKVSLRK	< 1	Cyt
M	gaccaagaggctattcaagatctct	gTAGC	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCTAVEEVSLRK	< 1	Cyt
N	gaccaagaggctattcaagatctct	gCCAC	gcag	t	ggaggaagtctctttaagaaaatag	286-DLCHAVEEVSLRK	< 1	Cyt
O	gaccaagaggctattcaagatctct	g	gcagCGTTTCC		aggaagtctctttaagaaaatag	286-DLWQRFQEVSLRK	< 1	Cyt
P	gaccaagaggctattcaagatctct	gTACCTTCC		t	ggaggaagtctctttaagaaaatag	286-DLCTFLEEVSLRK	< 1	Cyt
Q	gaccaagaggctattcaagatctct	g	gcagAGGA	t	ggaggaagtctctttaagaaaatag	286-DLWQRMEEVSLRK	< 1	Cyt

Mutations A to F correspond to those previously described by Falini et al³; mutation Q corresponds to mutation Mm described by Schnittger et al.⁶ Lowercase letters in nucleotide sequences indicate wild-type sequence residues; capital letters, mutated nucleotides. Underlined text indicates leucine-rich NES motif; italics, tryptophan (W) residues.

IH indicates immunohistochemistry; WT, wild type; Nucl, nuclear; Cyt, cytoplasmic.

NM_002520.

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