NOD/SCID-β2-microglobulin-/- engraftment data for human CD3- SP and CD3- non-SP LD mPB cells
Cells transplanted, no. of cells per mouse . | Proportion of repopulated mice . | . | |
|---|---|---|---|
| . | STRC-Ms . | STRC-MLs . | |
| CD3- non-SP | |||
| Experiment no. 1 | |||
| 1.2 | 0/3 | 3/3 | |
| 2.12 | 0/3 | 2/3 | |
| 8.6 | 0/3 | 2/3 | |
| 28.7 | 2/2 | 4/4 | |
| Experiment no. 2 | |||
| 4.0 | 0/3 | 1/3 | |
| 1.0 | 0/2 | 0/1 | |
| 42.0 | 0/3 | 1/3 | |
| Experiment no. 3 | |||
| 1.2 | 0/3 | 1/2 | |
| 2.8 | 1/3 | 1/3 | |
| 11.2 | 3/3 | 2/3 | |
| Experiment no. 4 | |||
| 2.6 | 0/3 | 0/3 | |
| 6.3 | 0/3 | 0/2 | |
| 25.0 | 0/3 | 0/3 | |
| Experiment no. 5 | |||
| 1.2 | 0/3 | 0/1 | |
| 5.1 | 1/2 | 0/2 | |
| 20.5 | 3/3 | 1/1 | |
| Experiment no. 6 | |||
| 2.5 | 0/3 | 2/3 | |
| 7.5 | 0/3 | 1/3 | |
| 30.0 | 0/3 | 0/2 | |
| Total | 10/56 | 21/49 | |
| CD3- SP | |||
| Experiment no. 1 | |||
| 0.52-12.5 | 0/13 | 0/13 | |
| Experiment no. 2 | |||
| 4.00-39.0 | 0/9 | 0/3 | |
| Experiment no. 3 | |||
| 3.90-29.67 | 0/9 | 0/7 | |
| Experiment no. 4 | |||
| 2.45-23.55 | 0/8 | 0/8 | |
| Experiment no. 5 | |||
| 0.42-3.60 | 0/9 | 0/7 | |
| Total | 0/48 | 0/38 | |
Cells transplanted, no. of cells per mouse . | Proportion of repopulated mice . | . | |
|---|---|---|---|
| . | STRC-Ms . | STRC-MLs . | |
| CD3- non-SP | |||
| Experiment no. 1 | |||
| 1.2 | 0/3 | 3/3 | |
| 2.12 | 0/3 | 2/3 | |
| 8.6 | 0/3 | 2/3 | |
| 28.7 | 2/2 | 4/4 | |
| Experiment no. 2 | |||
| 4.0 | 0/3 | 1/3 | |
| 1.0 | 0/2 | 0/1 | |
| 42.0 | 0/3 | 1/3 | |
| Experiment no. 3 | |||
| 1.2 | 0/3 | 1/2 | |
| 2.8 | 1/3 | 1/3 | |
| 11.2 | 3/3 | 2/3 | |
| Experiment no. 4 | |||
| 2.6 | 0/3 | 0/3 | |
| 6.3 | 0/3 | 0/2 | |
| 25.0 | 0/3 | 0/3 | |
| Experiment no. 5 | |||
| 1.2 | 0/3 | 0/1 | |
| 5.1 | 1/2 | 0/2 | |
| 20.5 | 3/3 | 1/1 | |
| Experiment no. 6 | |||
| 2.5 | 0/3 | 2/3 | |
| 7.5 | 0/3 | 1/3 | |
| 30.0 | 0/3 | 0/2 | |
| Total | 10/56 | 21/49 | |
| CD3- SP | |||
| Experiment no. 1 | |||
| 0.52-12.5 | 0/13 | 0/13 | |
| Experiment no. 2 | |||
| 4.00-39.0 | 0/9 | 0/3 | |
| Experiment no. 3 | |||
| 3.90-29.67 | 0/9 | 0/7 | |
| Experiment no. 4 | |||
| 2.45-23.55 | 0/8 | 0/8 | |
| Experiment no. 5 | |||
| 0.42-3.60 | 0/9 | 0/7 | |
| Total | 0/48 | 0/38 | |
Decreasing numbers of CD3- SP (× 105) and CD3- non-SP (× 102) LD mPB cells were transplanted into sublethally irradiated NOD/SCID-β2-microglobulin-/- mice in 6 independent experiments. The STRC-M and STRC-ML activity of the cells injected was detected by FACS of human cells present in the murine bone marrow 3 (STRC-Ms) and 8 (STRC-MLs) weeks later. A positive mouse was defined as one containing more than 10 human CD45/71+ cells in 20 000 PI- cells.