Effect of irradiation dose on the level of chimerism
. | . | Donor chimerism in chimeric mice 12-16 weeks after Tx, % . | . | . | ||
|---|---|---|---|---|---|---|
| Donor → recipient, irradiation dose, Gy . | Long-term donor chimerism (%) . | GR-1 . | B220 . | CD3 . | ||
| B6 → BALB/c | ||||||
| 6 | 3 of 10 (30) | 47.6 ± 47.9 | 59.3 ± 38.3 | 22.3 ± 11.9 | ||
| 6.5 | 4 of 5 (80) | 99.8 ± 0.2 | 99.9 ± 0.1 | 68.0 ± 8.1 | ||
| 7 | 5 of 5 (100) | 99.4 ± 0.5 | 99.0 ± 0.9 | 89.1 ± 8.4 | ||
| BALB/b → B6 | ||||||
| 4 | 0 of 6 (0) | ND | ND | ND | ||
| 5 | 3 of 6 (50) | 85.6 ± 11.4 | 60.1 ± 33.6 | 13.7 ± 14.0 | ||
| 6 | 5 of 5 (100) | 89.3 ± 22.2 | 96.8 ± 1.3 | 76.4 ± 3.1 | ||
. | . | Donor chimerism in chimeric mice 12-16 weeks after Tx, % . | . | . | ||
|---|---|---|---|---|---|---|
| Donor → recipient, irradiation dose, Gy . | Long-term donor chimerism (%) . | GR-1 . | B220 . | CD3 . | ||
| B6 → BALB/c | ||||||
| 6 | 3 of 10 (30) | 47.6 ± 47.9 | 59.3 ± 38.3 | 22.3 ± 11.9 | ||
| 6.5 | 4 of 5 (80) | 99.8 ± 0.2 | 99.9 ± 0.1 | 68.0 ± 8.1 | ||
| 7 | 5 of 5 (100) | 99.4 ± 0.5 | 99.0 ± 0.9 | 89.1 ± 8.4 | ||
| BALB/b → B6 | ||||||
| 4 | 0 of 6 (0) | ND | ND | ND | ||
| 5 | 3 of 6 (50) | 85.6 ± 11.4 | 60.1 ± 33.6 | 13.7 ± 14.0 | ||
| 6 | 5 of 5 (100) | 89.3 ± 22.2 | 96.8 ± 1.3 | 76.4 ± 3.1 | ||
BALB/c mice underwent irradiation with 6, 6.5, or 7 Gy (day 1) and underwent transplantation with 10 × 106 B6-depleted BM cells (day 0). B6 recipient mice were exposed to 4, 5, or 6 Gy (day 1) and underwent transplantation with 10 × 106 BALB/b BM cells (day 0). Donor chimerism was determined by flow cytometry 12 to 16 weeks after transplantation.
Tx indicates transplantation; ND, not determined.