Summary of established tumor cell lines
| Tumor line . | Doubling time, d . | Growth constant, k . | Pertinent markers* . |
|---|---|---|---|
| MML-12933 | 2.36 | 0.2931 | CD16, CD25, CD172a |
| LCL-13271 | 0.984 | 0.7042 | CD2, CD25, anti-mu heavy chain |
| ML-13381 | 1.65 | 0.4192 | CD25, CD172a, anti-mu heavy chain, anti-kappa light chain |
| CML-14736 | 2.295 | 0.302 | CD9, CD 16, CD25, CD172a |
| CML-15433 | 2.25 | 0.3078 | CD25, CD172a, anti-kappa light chain |
| LCL-15446 | 1.57 | 0.4416 | CD25, CD172a |
| LCL-17016L† | 1.99 | 0.3487 | CD25, anti-kappa light chain |
| LCL-17016P† | 0.52 | 1.324 | CD2, CD25, CD172a |
| LCL-17018 | 0.76 | 0.9128 | CD2, CD25, CD172a, anti-kappa light chain |
| Tumor line . | Doubling time, d . | Growth constant, k . | Pertinent markers* . |
|---|---|---|---|
| MML-12933 | 2.36 | 0.2931 | CD16, CD25, CD172a |
| LCL-13271 | 0.984 | 0.7042 | CD2, CD25, anti-mu heavy chain |
| ML-13381 | 1.65 | 0.4192 | CD25, CD172a, anti-mu heavy chain, anti-kappa light chain |
| CML-14736 | 2.295 | 0.302 | CD9, CD 16, CD25, CD172a |
| CML-15433 | 2.25 | 0.3078 | CD25, CD172a, anti-kappa light chain |
| LCL-15446 | 1.57 | 0.4416 | CD25, CD172a |
| LCL-17016L† | 1.99 | 0.3487 | CD25, anti-kappa light chain |
| LCL-17016P† | 0.52 | 1.324 | CD2, CD25, CD172a |
| LCL-17018 | 0.76 | 0.9128 | CD2, CD25, CD172a, anti-kappa light chain |
A total of 9 porcine tumor cell lines have been established from our MHC-defined miniature swine. Of these lines, 3 are derived from animals that developed spontaneous myeloid leukemias, while the remaining 6 lines were established from animals with lymphoma or posttransplantation lymphoproliferative disease (PTLD).
Growth constant, k, equals ln 2/T, where T is doubling time in days.
Full panel includes the following markers: negative IgG2a, MHC I, MHC II-DR, MHC II-DQ, CD1, CD2, CD3, CD4, CD5, CD8, CD9, CD16, CD21, CD25, CD172a, anti-mu heavy chain, and anti-kappa light chain. All tumor lines were found to be positive for MH.
LCL-17016L and LCL-17016P were both established from the same animal, but from different primary tissues. After growth in vitro, the cells derived from these tissues had different growth rates and surface marker patterns.